复方尼尔雌醇片预防去卵巢大鼠骨质疏松的实验研究  

Experimental study of compound nylestriol tablet on rats with osteoporosis induced by ovariectomy

作　　者：廖慧娟[1] 廖二元[1] 伍贤平[1] 刘锡玖 孙瑞元[2] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙410011 [2]皖南医学院药物研究所

出　　处：《中国骨质疏松杂志》2003年第1期57-60,共4页Chinese Journal of Osteoporosis

摘　　要：目的　探讨复方尼尔雌醇片 (Compoundnylestrioltablet,CNT ;每片含尼尔雌醇 0 5mg ,左炔诺酮 0 15mg)对去卵巢 (OVX)大鼠骨质疏松的预防作用。方法　建立OVX大鼠骨质疏松模型 ,分组分剂量予以CNT ,尼尔雌醇和左炔诺酮治疗 14周 ,观察其对全身、左股骨骨密度校正指数 (ADJB MD ;adjustedbonemineraldensity) ,骨Ca、P和血清Ca、P、ALP的影响。结果　CNT0 0 3 9～ 0 3 9mg/kg可增加全身及离体左股骨ADJBMD ,且具有剂量依赖性 ;CNT 0 117～ 0 3 9mg/kg可增加骨Ca含量 ,增高血Ca水平 (P <0 0 5或P <0 0 1)。单用CNT中所含相应剂量的尼尔雌醇 ,也可增加ADJBMD ,增加骨Ca含量及增高血Ca水平的作用 ,但程度不如CNT。结论　CNT对OVX大鼠骨质疏松模型有预防作用 。Objective To evaluate the effect of compound nylestriol tablet (CNT) which contains 0 5 mg of nylestriol(NYL) and 0 15 mg of levonorgestrel(LNG) per tablet on preventing osteoporosis in OVX rats Methods Four month old SD rats were allotted to 12 groups( n =10 each) Sham operated and control OVX groups received vehicle,whereas other OVX groups received respectively CNT(0 039 mg/kg,0 117 mg/kg,0 390 mg/kg qod orally),NYL (0 03 mg/kg,0 09 mg/kg,0 30 mg/kg qod orally), LNG(0 009 mg/kg,0 027 mg/kg,0 090 mg/kg qod orally) and ethinylestradiol (0 014 mg/kg qod orally) for 14 weeks Whole body adjusted bone mineral density (ADJBMD),left femoral ADJBMD in vitro ,serum calcium (Ca)?phosphorus(P)?alkaline phosphatase(ALP),bone Ca?P were measured Results 0 039 0 39 mg/kg CNT increased whole body ADJBMD and left femoral ADJBMD dose dependently Bone and serum Ca enhanced at 0 117-0 39 mg/kg ( P <0 05 or P <0 01) Single use of nylestriol at the dose corresponding to that contained in CNT increased the ADJBMD, bone Ca and seram Ca as well,but did less than that of CNT Conclusion CNT prevents bone loss in OVX rats,which is better than nylestriol alone or levonorgestrel does

关 键 词：复方尼尔雌醇片 预防 去卵巢大鼠 骨质疏松 实验研究 

分 类 号：R681[医药卫生—骨科学] R977.12[医药卫生—外科学]