丝素蛋白膜上v Wf抗体的固定化及其体外抗凝血性能  

Immobilization of v Wf Antibody on Fibroin Membrane and Its AnticoagulantPerformance in vitro

作　　者：高运华[1] 杨新林[1] 范翠红[1] 李茂林[1] 朱鹤孙[1] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081

出　　处：《高技术通讯》2003年第3期56-59,共4页Chinese High Technology Letters

基　　金：973规划 (G19990 6 470 5 )资助项目。

摘　　要：用凝血因子的抗体对生物材料进行表面改性以提高其抗凝血性能。以丝素蛋白膜为基质 ,利用等离子体处理辅助的共价交联方法对vWf因子 (vonWillebrandfactor)抗体进行了固定化。用酶联免疫法和抗体过剩法对固定化效果进行了评价 ,固定化抗体的活性采用体外凝血时间 (APTT ,TT和PT)测定进行检测。结果显示 ,通过这种方法可以有效地将vWf抗体固定化 ,丝素蛋白膜固定化vWf抗体后 ,其抗凝血性能有了一定的改善。本研究结果拓宽了抗体固定化技术的应用范围 ,同时为抗凝血材料的设计提供了一种新的思路。This study aimed at modifying the surface of a biomaterial by the utilization of the antibodies in order to improve the anticoagulant property of the biomaterial. The vWf (von Willebrand factor)antibodies were immobilized on the surface of Bombyx mori silk fibroin membrane by NH 3 plasma treatment followed by covalent cross-linking reaction. The immobilization efficiency was evaluated by two methods including the enzyme-linked immunosorbent assay (ELISA) and antibody surplus. The in vitro antithrombogenicity representing the activity of immobilized vWf antibody was determined by the method of activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) test. The results demonstrate that the vWf antibodies are immobilized on silk fibroin membranes in an efficient and activity-retaining form and hence, the anticoagulant performance of modified fibroin membrane is improved. These results enlarge the application of antibody immobilization technique, and provide a new idea about the design of anticoagulant materials.

关 键 词：体外抗凝血性能 丝素蛋白膜 vWf抗体 固定化 抗凝血材料 生物材料 凝血因子 

分 类 号：R318.08[医药卫生—生物医学工程]