葡萄籽原花青素提取物抗氧化作用研究  

Studies on the Antioxidation Effects of Grape Seed Procyanidins Extract

作　　者：朱振勤[1] 翟万银[2] 陈季武[1] 夏晶[1] 傅蓓蓓[1] 谢萍[1] 胡天喜[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]中国科学院硅酸盐研究所生物材料与组织工程中心,上海200050

出　　处：《华东师范大学学报（自然科学版）》2003年第1期98-102,共5页Journal of East China Normal University(Natural Science)

摘　　要：　为研究葡萄籽原花青素提取物(grapeprocyanidinsextract,GPE)抗氧化和对DNA氧化损伤的保护作用,我们采用七种生物化学发光体系,检测了GPE对O·2、·OH、H2O2、ONOO-和全血嗜中性白细胞"呼吸爆发"产生的多种活性氧的清除作用,以及对·OH引起的DNA氧化损伤的保护作用;采用Fe2+诱发脂蛋白多不饱和脂肪酸过氧化比色体系,检测了GPE对脂质过氧化的抑制作用。结果显示,GPE能有效地清除O·2、·OH、H2O2、ONOO-和全血嗜中性白细胞"呼吸爆发"产生的多种活性氧而抑制体系发光,并有效抑制脂质过氧化,半抑制浓度分别约为0.2μg ml、90μg ml、0.5μg ml、10μg ml、130μg ml及70μg ml;25μg ml的GPE对DNA氧化损伤的抑制率约为70%。提示GPE能有效清除多种自由基,保护DNA免受·OH引起的氧化损伤,是良好的抗氧化剂。In order to study the antioxidation effects of grape seed procyanidins extract (GPE), seven chemiluminescence systems were used to determine the protective effect of GPE on DNA damage caused by ·OH and the scavenging effect of GPE on O·2,·OH, H2O2 and ONOO-, and various reactive oxygen species produced during 'respiration burst' of whole blood phagocytes. The inhibiting effect of GPE on lipid peroxidation was evaluated by using the colorimetric system of lipoprotein polyunsaturated fatty acids peroxidation induced by Fe2+. The experiments showed that GPE can effectively scavenge O·2, ·OH, H2O2 , ONOO- and various reactive oxygen species produced during 'respiration burst' of whole blood phagocytes, and inhibit lipid peroxidation (IC50s are 0.2μg /ml, 90μg /ml, 0.5μg /ml, 10μg /ml, 130μg /ml and 70μg /ml respectively); the inhibiting rate of DNA oxidative damage is approximately 70%, when GPE at a concentration of 25μg /ml is given. These results indicate that GPE is an efficient antioxidant and can effectively scavenge various free radicals and provide effective protection against DNA damage caused by ·OH.

关 键 词：葡萄籽原花青素 活性氮 DNA氧化损伤 抗氧化剂 

分 类 号：Q591[生物学—生物化学] Q599