塞来昔布抑制慢性支气管炎大鼠气道粘蛋白高分泌的实验研究  

A study on inhibition of celecoxib on the hypersecretion of airway mucin in chronic bronchitis rats

作　　者：黄勇[1] 杨肇亨[1] 周向东[2] 刘维会[1] 段绪伟[1] 

机构地区：[1]第三军医大学大坪医院呼吸内科,重庆400042 [2]重庆医科大学第二临床学院肺科,重庆400010

出　　处：《重庆医科大学学报》2003年第2期174-178,共5页Journal of Chongqing Medical University

摘　　要：目的 :探讨塞来昔布抑制慢性支气管炎大鼠气道粘蛋白高分泌的作用。方法 :Wistar大鼠气管内注入 0 .1%脂多糖2 0 0 μl复制模型 ,采用图象分析、ELISA等方法检测病理学改变、支气管肺泡灌洗液 (BALF)中粘蛋白量的变化。 结果 :注入脂多糖 3周后出现慢性支气管炎的病理学表现 ,包括杯状细胞增生、粘膜下腺体增生肥大 ,分泌细胞内及BALF中粘蛋白量增加 ;塞来昔布可以显著减少每 1mm气管上皮中杯状细胞数目、上皮及腺体细胞粘蛋白面积 %与未分泌指数、BALF中粘蛋白含量。结论 :塞来昔布通过特异性地抑制环氧化酶 2的活性 ,可以减少慢性支气管炎大鼠气管上皮杯状细胞增生 ,也可抑制杯状细胞和粘液腺体粘蛋白的合成分泌亢进。Objective: To investigate the inhibition of celecoxib on the hypersecretion of airway mucins in the chronic bronchitis rats. Methods: Rat chronic bronchitis model was established by intratracheal instillation of 0.1% lipopolysaccharide 200μl.With image analysis and ELISA,the changes of pathology and mucins in bronchoalveolar lavage fluid(BALF)were observed. Results: The rats shared many features of chronic bronchitis after instillation,including goblet cells hyperplasia,submucosal glands hyperplasia and hypertrophy,mucins overabundance of secretory cells and BALF.Celecoxib could remarkably decrease the number of goblet cells per 1mm epithelium,mucins area% and pre-secretion index of glands and epithelia,mucins in BALF. Conclusion: Through inhibiting specifically cyclooxygenase 2,celecoxib could decrease goblet cells hyperplasia,inhibit excessive mucin synthesis and secretion in goblet cells and mucus glands of chronic bronchitis rats.

关 键 词：塞来昔布 慢性支气管炎 粘蛋白 

分 类 号：R562.21[医药卫生—呼吸系统]