抗藻酸盐血清对黏液型铜绿假单胞菌生物被膜杀菌活性的影响  

The influence of anti-bacterial activity of the anti-alginate serum on the mucoid Pseudomonas aeruginosa biofilm

作　　者：裴斐[1] 王睿[1] 柴栋[1] 方翼[1] 张梅[2] 

机构地区：[1]解放军总医院临床药理研究室,北京100853 [2]上海第二军医大学药学院,上海200433

出　　处：《中国新药杂志》2003年第3期189-191,共3页Chinese Journal of New Drugs

基　　金：国家自然科学基金资助课题 ( 30 0 70 72 0 )

摘　　要：目的 :研究抗藻酸盐血清与加替沙星联用对黏液型铜绿假单胞菌生物被膜杀菌活性的影响。方法 :采用MTT方法测定黏液型铜绿假单胞菌生物被膜活菌数 ,用微量稀释法测定抗菌药物最低生物被膜清除浓度(MBEC)。结果 :1∶1抗藻酸盐血清与 1×MIC ,4×MIC和 8×MIC加替沙星联用可以使细菌生物被膜中的活菌数量 (单位为lgcfu·cm-2 )分别从 ( 6 .6 3± 0 .85 ) ,( 6 .48± 1.12 )和 ( 5 .73± 1.10 )显著降低至 ( 5 .76± 0 .90 ) ,( 5 .0 2±0 .93)和 ( 4.70± 0 .6 2 ) ,使加替沙星对铜绿假单胞菌的MBEC从 8μg·mL-1降低至 4μg·mL-1。结论 :抗藻酸盐血清与加替沙星联用后 ,能够增强抗菌药物对黏液型铜绿假单胞菌生物被膜的杀菌活性。Objective:To investigate the influence of anti-bacterial activity of the anti-alginate serum on the mucoid Pseudomonas aeruginosa biofilm.Methods:The viable counts of bacteria in biofilm were detected by MTT method.The minimal biofilm eradication concentration(MBEC) was determined by micro-dilution method.Results:The 1∶1 dilution anti-alginate serum decreased viable counts(lgcfu·cm -2 ) in biofilms significantly from(6.63±0.85),(6.48±1.12) and (5.73±1.10) to (5.76±0.90),(5.02±0.93) and (4.70±0.62) when it combined with 1×MIC,4×MIC and 8×MIC gatifloxacin respectively.1∶1 dilution anti-alginate serum decreased the MBEC from 8μg·mL -1 to 4μg·mL -1 of gatifloxacin.Conclusion:The anti-alginate serum could enhance the activity of gatifloxacin.

关 键 词：黏液型铜绿假单胞菌 细菌生物被膜 抗藻酸盐血清 加替沙星 

分 类 号：R976[医药卫生—药品]