棉花抗黄萎病基因的QTL定位  

QTL Mapping of Genes Resistant to Verticillium Wilt in Cotton

作　　者：高玉千[1] 聂以春[1] 张献龙[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《棉花学报》2003年第2期73-78,共6页Cotton Science

基　　金：国家自然科学基金(39830240);国家"863"项目(2001AA211121);国家"948"项目(201012)资助

摘　　要：以高感黄萎病的陆地棉品种"邯郸208"与高抗黄萎病海岛棉品种"Pima90"的136个F2单株为作图群体,构建了一个包括17个连锁群、标记间平均间距18.61cM、全长1842.8cM的陆海种间分子标记遗传连锁图,该图约覆盖棉花基因组的36.8%。单因子方差分析和复合区间作图检测到与黄萎病抗性相关的3个QTL,分别位于第四连锁群和第七连锁群上,分别解释表型变异方差的15.39%、54.11%和57.18%。初步认为海岛棉"Pima90"对陆地棉"邯郸208"的黄萎病抗性由两个主效QTL和一个微效QTL共同控制。A genetic linkage map was constructed using 136 F2 individuals developed by a cross of Gosssypium hirsutum cv. Handan208×G. barbadense cv. Pima90. The linkage map comprised 99 loci mapped to 17 linkage groups with an average distance of 18.61 cM between markers, covering 1842.8cM, or approximately 36.8% of the total cotton genome. This map was used to map the lesion length of vascular bundle, which is a factor associate with Verticillium wilt, by both onefactor analysis and composite interval methods. Three QTLs were detected on two linkage groups, and explained 15.39%, 54.11% and 57.18% of the phenotypic variance, respectively, which might mean that the resistance of V. wilt is controlled by two major QTLs and one minor QTL.

关 键 词：棉花 黄萎病 分子标记遗传连锁图 QTL定位 

分 类 号：S562.034[农业科学—作物学]