黑龙江省骨干玉米自交系愈伤组织的诱导及植株再生  

Callus induction and regeneration of inbred lines used largely in Heilongjiang

作　　者：肖莉杰[1] 苍晶[1] 徐仲[1] 侯艳华[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2003年第1期63-67,共5页Journal of Northeast Agricultural University

基　　金：国家植物转基因研究与产业化"专项课题资助

摘　　要：选择取了 8个基因型的黑龙江省主栽玉米自交系 ,以幼胚为外植体诱导愈伤组织探讨了基因型、胚龄及幼胚大小、2 ,4 - D浓度对愈伤组织诱导的影响。结果表明 ,基因型是影响幼胚培养的主要因素 ,它决定了愈伤组织的诱导率、状态和类型及分化率 ;幼胚大小以 1～ 2 m m时诱导效果为最佳 ;2 ,4 - D是愈伤诱导的必需外源激素 ,浓度范围为 1～ 3mg· L- 1 ;幼胚的接种方式、夹碎幼胚、Ag NO3的添加一定程度上影响愈伤组织的产生 ;不同基因型继代培养及分化有差异 ;Factors influencing the callus induction of immature embroys such as genotype,length of immature embroys,concentration of 2,4 D were studied,by using immature embroys from eight imbred lines used largely in agriculture production in Heilongjiang Province.The results showed that genotype was the major objective in callus induction,immature embryos of 1.0～2.0 mm in length were best,and we suggest the suitable concentration of 2,4 D was 1～3 mg·L -1 .Other factors affecting callus induction were also discussed,and established relatively preliminary regeneration system of maize.$$$$

关 键 词：玉米 幼胚 愈伤组织 再生 

分 类 号：S513[农业科学—作物学]