引物对甘薯品种聚类分析结果的影响  

The Effection of Primer on Cluster Analysis of Ipompea batatas LAM.

作　　者：孙勇[1] 马代夫 谢逸萍 蒋继宏[1] 陈凤美[1] 

机构地区：[1]徐州师范大学江苏省药用植物重点实验室,江苏徐州221116 [2]徐州农科所甘薯研究中心,江苏徐州221121

出　　处：《徐州师范大学学报（自然科学版）》2003年第1期53-57,共5页Journal of Xuzhou Normal University(Natural Science Edition)

基　　金：江苏省高技术计划基金项目(BG2002311);国家"863"高技术发展计划基金项目(2001AA241181)

摘　　要：应用RAPD技术对10个供试甘薯品种的遗传关系进行了研究,探讨引物数量、多态性率和所用引物带型丰 富度对聚类分析结果的影响.结果表明:聚类分析准确度与引物数量、引物的带型数有关,多态性率不是选用引物的主要 标志.因此,在利用RAPD技术研究物种间遗传关系时,有必要保证一定数量的引物,且引物具有较多的带型.RAPD technique is used to study the genetic relationships among the ten tested strains of Ipompea batatas Lam. and the impact on the cluster analysis brought by the number of primers, polymorphism rate and the number of RAPD bands. It shows that the precision of the cluster analysis is revelant with the number of the primers and the number of RAPD bands, furthermore,the polymorphism rate of primers isn t the important factor on result. While RAPD technique is used to study the genetic relationship among strains, it should be assured that there are certain amount of primers and certain number bands of the primers.

关 键 词：甘薯 随机扩增多态性DNA 引物 聚类分析 

分 类 号：S531.032[农业科学—作物学]