人SCF基因转染NK细胞系的生物学特性研究  

The Characterization of NK-92 Cell Line Transfected with Human Stem Cell Factor Gene

作　　者：张建[1] 张建华 许晓群 冯进波 孙汭[1] 田志刚[1] 

机构地区：[1]中国科技大学免疫学研究所,合肥230027 [2]山东省医科院基础所,济南250062

出　　处：《中国肿瘤生物治疗杂志》2003年第1期21-24,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家高技术研究发展专项经费资助(编号 2 0 0 2AA2 1615 1)

摘　　要：目的 :研究人SCF基因转染NK细胞系的生物学特性。方法 :利用LipofecTAMINE将SCF真核表达载体 pcD NA3 hSCF转染NK 92细胞系 ,RT PCR鉴定表达SCF的细胞克隆并以SCF依赖细胞株TF 1测活 ,MTT法检测增殖和杀伤活性。流式细胞术检测细胞表面分子CD3,CD16和CD5 6。结果 :建立表达hSCF基因的NK 92 hSCF细胞株 ,在IL 15培养条件下 ,其增殖能力显著高于NK 92 ,并且低剂量的IL 15可以维持其较高的杀伤活性流式细胞术检测细胞表面分子CD3、CD16和CD5 6。结论 :可以通过SCF基因修饰NK 92 ,改变其生物学特性 ,提高其增殖能力 。Objective: To study the characterization of NK cell line transfected with hSCF gene.Methods: pcDNA3 hSCF was transfected into NK 92 cell line with LipofectAMINE ,RT PCR was used to identify NK 92 hSCF cell which express hSCF and the activity was assayed by TF 1 cell line. CD3, CD16 and CD56 molecules were tested by FACS. Results: We established NK 92 hSCF cell line which express hSCF steadily, and its proliferation increased compared with parental cell line when incubated with rhIL 15 or rhIL 2. Conclusion: The characterization of NK 92 could be changed by transfecting with hSCF gene, and it′s proliferation was improved, The gene transfection of NK 92 cell made it suitable for clinical applications.

关 键 词：造血干细胞因子 转染 NK细胞系 生物学特性 

分 类 号：R392.12[医药卫生—免疫学]