组蛋白H2A与染色质的动态结合  

Dynamic Association of Histone H2A and Chromatin

作　　者：郭焱[1] 孙玉慧[1] 魏朝亮[1] 丁明孝[1] 陈建国[1] 

机构地区：[1]北京大学生命科学学院细胞生物学及遗传学系,北京100871

出　　处：《北京大学学报（自然科学版）》2003年第2期182-186,共5页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家重点基础研究发展规划基金资助项目 (G19990 5 390 6 )

摘　　要：在真核细胞中 ,核小体的核心结构组蛋白被DNA紧密缠绕 ,但是这种紧密的结合有时也可能变得松散 ,以利于其他蛋白与DNA的结合。将组蛋白H2A与绿色荧光蛋白的融合表达载体和核仁纤维蛋白 (fibrillarin)与红色荧光蛋白的融合表达载体共转染COS 7细胞 ,利用荧光漂白恢复(FRAP)技术研究了H2A在细胞核内的动态变化。结果显示 ,在少数间期细胞的核仁区有组蛋白聚集现象 ,且此区域内H2A的运动速度显著地高于非核仁区的H2A ,推测该现象仅出现于细胞周期的某个时期。Histone and DNA bind together to form the nucleosome,but this tight binding can loose at some times to facilitate other proteins to interact with DNA.The authors constructed H2A pEGFP and fibrillarin-pDsRed 1 expression vectors,and studied the dynamics of H2A,using fluoresence recovery after photobleaching (FRAP) techniques.It was showed that in a small number of interphase cells cultured in vitro, H2A not only distributed in the nucleoplasm,but also congregated in the nucleolar area.The FRAP analysis indicated that H2A in the nucleolar area moved remarkably faster than that in the nucleoplasm.It supposed that nucleolus may be as a pool of histone H2A at some special time during cell cycle.

关 键 词：组蛋白 核仁 荧光漂白恢复 

分 类 号：Q343[生物学—遗传学]