氦氖激光照射对小鼠脾淋巴细胞增殖反应影响的试验研究  

Study on the effects of He-Ne laser irradiation on the splenic T lymphocyte proliferative responses in mice

作　　者：黄保续[1] 刘焕奇[1] 刘云[1] 曲志娜[2] 刘西锋[1] 程绍辉[1] 王洪斌[1] 高利[1] 王海彬[1] 

机构地区：[1]东北农业大学动物医学院,哈尔滨150030 [2]农业部动物检疫所,青岛266032

出　　处：《激光杂志》2003年第2期69-71,共3页Laser Journal

基　　金：国家自然科学基金资助项目 (项目批准号 :3 0 170 712 )

摘　　要：目的 :研究氦氖激光照射对小鼠脾淋巴细胞增殖反应的影响。方法 :以BALB c小鼠为研究对象 ,应用 7.3 3 ,11.0 0 ,14 .67,2 2 .0 0和3 6.67J cm2 五种剂量的氦氖激光照射小鼠哈德腺 ,连续照射 8d ,并于照射开始后第 3、6、9、13和第 17d动态监测实验鼠脾淋巴细胞增殖反应。结果 :7.3 3 ,11.0 0 ,14 .67和 2 2 .0 0J cm2 四个剂量组均可增强脾淋巴细胞增殖反应 ,但 11.0 0 ,14 .67和 2 2 .0 0J cm2 三个剂量组在第 3d就达到峰值 ,此后逐步下降 ,而 7.3 3J cm2 剂量组直到第 9d才达到峰值 ,且维持时间最短。与此相反的是 ,3 6.67J cm2 剂量组则在第 3d就出现抑制效应 ,直至第 13d才恢复至正常水平。结论 :适当剂量的氦氖激光照射可对小鼠脾淋巴细胞增殖反应产生增强效应 ,而过大剂量He -Ne激光则对免疫机能产生抑制效应。Objective:The effects of He-Ne Laser Irradiation on splenic T lymphocyte proliferative responses in mice were studied,in order to illustrate mechanism of the immune modulation effect of He-Ne laser irradiation in mice.Method:By irradiating upon the Harder's glands of the mice with He-Ne laser at five different dosages of 7.33,11.00,14.67,20.00 and 36.67J/cm 2 for eight days,the splenic T lymphocyte proliferative responses were monitored kinetically at the 3rd,6th,9th,13th and 17th day after laser irradiation.Results:7.33,11.00,14.67 and 22.00J/cm 2 He-Ne Laser increased the values of splenic T lymphocyte proliferative responses (P<0.01 or P<0.05),while the values decreased significantly in the group of 36.67J/cm 2.Conclusion:He-Ne laser with the proper dosages showed up regulation effects on the splenic T lymphocyte proliferative responses in mouse;while the large dose showed down regulation effects.

关 键 词：氦氖激光 免疫调节效应 小鼠 脾淋巴细胞增殖反应 

分 类 号：R551.2[医药卫生—血液循环系统疾病]