鸡贫血病毒vp3基因克隆、序列分析和比较  

Cloning,Sequencing and Comparing of vp3 Gene of Chicken Anemia Virus

作　　者：丁乃峥[1] 何成强[1] 李云龙[2] 李景鹏[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]山东师范大学生物系,山东济南250014

出　　处：《中国兽医学报》2003年第2期130-133,共4页Chinese Journal of Veterinary Science

基　　金：黑龙江省科委攻关项目

摘　　要：克隆了从我国哈尔滨分离的一株鸡贫血病毒 (CAV)的 vp3基因 ,并对之进行了测序。该基因的开放读码框由36 6 bp组成 ,编码 12 1个氨基酸 ,氨基酸组成具有已报道 VP3的典型特点。本次克隆的基因与 Gen Bank收录的 CAV的 vp3基因进行序列比较 ,同源性至少为 98%。与国内报道的山东株 SJ1的 vp3基因有 3个核苷酸的差异 ,表明国内的 CAV毒株已经产生了一些分化。在 EMBL 中比较本次克隆的 VP3蛋白一级结构 ,与之差异最大的是马来西亚分离株的 VP3,有 5个氨基酸残基不同 ,同源性为 96 %。同时收集 EMBL 中的 CAV的 VP3蛋白绘制进化树 ,我国哈尔滨分离的 CAV毒株与 CIA进化关系最近 ,而与 Cux- 1的 2个衍生株 QDWX1、QDWX3进化关系最远。这些结果进一步证明了 CAV在遗传方面是较保守的病毒 ,来自哈尔滨的 CAV不是 CAV的一个独立分支。The vp3 gene of chicken anemia virus was amplified by polymerase chain reaction from liver of chicken infected by chicken anemia virus in Harbin of China and cloned into pUC18.The recombinant plasmid was identified by restriction digestion.The sequence of the gene was analyzed with F promer by TaKaRa Biotechniques(Dalian,China).Comparing the gene with all genes in GenBank and Shandong strain SJ1 of China,It was found that it had well homology to the reported vp3 genes in GenBank.Amino sequence of the VP3 protein was analyzed with alignment in EMBL and the genetree is drawn with BLAST2 & Orthologue.It was found that the VP3 protein and VP3 of other CAVs were identical from 96% to 100% of amino acid positions.Results confirmed the limited genetic variability of CAV and the CAV isolated in Harbin is not found on a new separate branch.

关 键 词：VP3基因 序列分析 鸡 贫血病毒 基因克隆 

分 类 号：S858.31[农业科学—临床兽医学] S852.65[农业科学—兽医学]