牙鲆群体生化遗传学研究——Ⅰ.组织特异性研究  

Biochemical genetics of paralichthys olivaceus population I. A study on tissue-specific patterns of allozymes

作　　者：黎中宝[1] 

机构地区：[1]集美大学水产学院,厦门361021

出　　处：《中国生态农业学报》2003年第2期1-4,共4页Chinese Journal of Eco-Agriculture

摘　　要：采用聚丙烯酰胺凝胶电泳技术,研究了AAT、EST、SOD、MDH、LDH和ME等位酶在牙鲆[Paralichthys olivaceus(T.et S.)]心脏、肌肉、鳃、眼睛、肾脏、肝脏、肠、脾、胃组织器官中表达,并分析了各种酶的等位酶位点表达以及酶谱表型。研究结果表明EST、SOD、MDH和ME 4种等位酶在不同组织中表达相同,AAT、LDH 2种等位酶在不同组织中表达差异,可能是受不同基因位点或同一基因位点不同等位基因控制的结果,与各组织完成特有生化代谢活动有关。The allozyme patterns of AAT,EST,SOD,MDH,LDH,and ME in heart,muscle,branchia,eye,kidney,liver,intestine,spleen and stomach of paralichthys olivaceus (T.et S.) were studied by using the assay of vertical slab polyacrylamide gel electrophoresis.The results indicate that four allozymes (EST,SOD,MDH,and ME) show the same expressions of allozyme in different tissues,and two allozymes (AAT,LDH) show the different expressions of allozyme in different tissues,which may be controlled by different gene loci or different alleles in the same gene locus,are closely related to the tissues-specific biochemical metabolism.

关 键 词：生化遗传学 牙鲆 组织特异性 群体遗传学 

分 类 号：Q959.4[生物学—动物学] S965.399[农业科学—水产养殖]