光系统Ⅱ核心天线CP47热稳定性的荧光动力学  

Fluorescence Dynamics on Thermal Stability of Inner Antenna CP47 in PS Ⅱ

作　　者：蔡霞[1] 贺俊芳[1] 王水才[1] 任兆玉[1] 刘晓[1] 彭菊芳[1] 匡廷云[2] 

机构地区：[1]中国科学院西安光机所瞬态光学技术国家重点实验室 [2]中国科学院植物研究所,北京100093

出　　处：《光子学报》2003年第3期257-260,共4页Acta Photonica Sinica

基　　金：国家重点基础研究专项经费 (G199880 10 10 0 );所长基金资助项目

摘　　要：采用激励光源为 4MHz、5 14.5nm的分幅扫描单光子计数荧光装置对从菠菜中分离提纯的核心天线CP4 7的热稳定性进行研究 ,得到经 2 0℃、4 2℃和 4 8℃处理后的动力学三维荧光谱 .它们在最大峰值处的时间常量及其chla分子发射荧光的效率分别为 :2 0℃ :2 .84 9ns( 4 3.60 % ) ,4 .0 2 4ns( 35 .37% ) ;4 2℃ :2 .10 7ns( 5 1.11% ) ,3.65 7ns( 37.5 9% ) ;4 8℃ :3.80 1ns( 36.69% ) ,4 .0 149ns ( 34 .68% ) .因此 ,在CP4 7中存在有 2组处于不同聚合状态的chla分子 ,3个处理温度中较短的时间组分属于 2组中处于同一种聚合状态色素分子之间chla→chla→chla基态的能量传递时间 ,较长的时间组分属于 2组处于不同聚合状态色素分子之间chla→chla→chla基态的能量传递时间 .CP4 7中 ,2 0℃～ 4 2℃之间的温度对蛋白质空间结构的影响较小 ,与蛋白质结合的 2种结合状态的chla分子之间的空间位置改变也较少 .而 4 2℃～ 4 8℃之间的温度引起较大的蛋白质空间结构和与蛋白质结合的 2种结合状态的chla分子之间的空间位置改变 .Thermal stability of inner antenna CP47 were studied by using fluorescence dynamics. CP47 were treated at 20 ℃, 42 ℃,48 ℃ respectively and their three-dimensional dynamic fluorescence spectrum were gotten.The fluorescence lifetime at maximum peak and chla emissiom efficiencies were: 20 ℃:2.849 ns(43.60%), 4.024 ns(35.37%);42 ℃;2.107 ns(51.11%),3.657 ns(37.59%);48 ℃:3.801 ns(36.69%),4.0149 ns (34.68%).Therefore, CP47 had two series of chla molecules under different conditions. The shotter lifetimes were the time needed by energy transportation from chla to chla ground state within two series of chla molecules, the longer ones were the time needed by energy transportation from chla to chla ground state between two series of chla molecules. In CP47, the protein conformation and chla spatial orientation were affected a little between 20 ℃ and 42 ℃. While, between 42℃ and 48℃, they had distinct changes.

关 键 词：热稳定性 CP47 核心天线 chla 动力学荧光谱 

分 类 号：Q63[生物学—生物物理学]