应用斑马鱼胚胎致畸效应检测环境污染物毒性  

Application of the Teratogenic Effects of Embryo Development in Zebrafish to the Determination of Toxicity of Environmental Pollutants

作　　者：朱琳[1] 史淑洁[1] 佟玉洁[1] 

机构地区：[1]南开大学环境科学与工程学院,天津300071

出　　处：《环境与健康杂志》2003年第2期122-124,共3页Journal of Environment and Health

基　　金：高等学校重点实验室访问学者基金资助项目

摘　　要：斑马鱼胚胎技术是利用鱼类胚胎发育初期较高的灵敏度,通过观察鱼受精卵经化合物染毒后的胚胎发育过程,分析评价此化合物的毒性作用方式、特效作用时间、胚胎毒性和致畸性。具有成本低、易操作,特别是可以同时分析多项指标的优点。该文着重介绍这一新技术的基本操作要领、技术特色及其在国内外化合物毒性效应测定上的广泛应用,并进一步根据我国目前最为迫切的环境问题,提出将该项技术应用于检测综合污水毒性的展望。The embryo development technique in Zebrafish, Brachydanio rerio, is a toxicity testing method making use of the high sensitivity of fish embryo development in early stage to study and evaluate the specific effecting mechanism, the most sensitive effecting time, embryo toxicity and teratogenicity of chemicals through observing the development process of zebrafish embryo after chemical exposure to fertilized ova. This technique has been widely used to test toxicity of chemicals with the advantages of low cost, high sensitivity, simple to operate and simultaneous to detect multi-endpoints. The main methodology, technical characteristics and the status of world-wide application of this technique are reviewed in this paper. Based on the urgent environmental problems in China, the prospects to use this method for monitoring toxicity of mixed pollutants in wastewater are put forward.

关 键 词：环境污染物 毒性实验 斑马鱼 致畸效应 

分 类 号：X83[环境科学与工程—环境工程] R991[医药卫生—毒理学]