胡萝卜光自养型愈伤组织的诱导培养及其光合特性  

PHOTOAUTOTROPHIC GROWTH AND PHOTOSYNTHETIC CHARACTERISTICS OF CARROT CALLUS

作　　者：曾小美[1] 王凌健[1] 夏镇澳[1] 沈允钢[1] 

机构地区：[1]中国科学院上海植物生理生态研究所,上海200032

出　　处：《实验生物学报》2003年第1期18-22,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家重点基础研究规划项目(No.G1998010100)

摘　　要：经诱导得到胡萝卜光自养型愈伤组织。以叶绿素为单位计算,获得的光自养型愈伤组织的光合活力达到甚至超过了整体植株叶片水平。同时测定愈伤组织光自养过程中光合特性的变化,结果表明其叶绿素含量逐渐上升、暗呼吸速率和Chl a/Chl b比值逐渐下降。并且用电子显微镜观察到愈伤组织中叶绿体结构逐渐发育的过程。Photoautotrophic carrot callus was induced by gradually lowering the sucrose concentration in culture medium. In the course of induction, the net photosynthetic rate on chlorophyll basis of callus increased gradually with the decreasing of sucrose concentration in culture medium, and the net photosynthetic rate of photoautotrophic callus could even be higher than that of leaf. In the course of changing into pholoaulotrophic callus, its chlorophyll content increased gradually, and the dark respiratory rate and the ratio of Chl a/Chl b decreased gradually. Parallel with these was the development of the microstructure of thylakoid of callus.

关 键 词：胡萝卜 光自养型愈伤组织 植物 诱导培养 光合特性 

分 类 号：Q945[生物学—植物学]