实时定量PCR方法检测鼻咽癌病人全血EB病毒拷贝数的实验研究  

REAL-TIME QUANTITATIVE PCR ASSAY OF EPSTEIN-BARR VIRUS IN WHOLE BLOOD CELLS OF NASOPHARYNGEAL CARCINOMA PATIENTS

作　　者：王海[1] 邓琳[1] 陈苏红[2] 王升启[2] 曹亚[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙,410078 [2]军事医学科学院二所,北京,100000

出　　处：《实验生物学报》2003年第1期32-36,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家重点基础研究发展规划(973)"恶性肿瘤发生发展的基础研究"(项目编号:G1998051201);国家自然科学基金杰出青年基金(项目编号:39525022)

摘　　要：EBV在多种肿瘤中存在,并在相关肿瘤的发病中起一定的作用.本文应用实时定量PCR方法检测鼻咽癌与健康对照全血EBV拷贝数.结果表明在73例鼻咽癌病人与83例正常对照中,NPC组中EBV阳性率为46.6%,对照组阳性率为13.3%.对照组平均EB病毒拷贝数为3.9×104拷贝/μgDNA,高于鼻咽癌组(1.7×105拷贝/μgDNA).全血EBV的感染与鼻咽癌的发生相关,而裂解状态EBV在细胞转化过程中的作用可能更大.应用实时定量PCR进行全血EBV的检测可用于鼻咽癌的分子诊断.EBV is detected in more and more tumors, and is relative to carcinogenesis. We studied the copies of EBV DNA in whole blood of NPC patients and healthy controls by real lime quantitative PCR In the 73 NPC patients and 83 controls, the positive rale of EBV in blood of NPC is 46.6% , while 13. 3% in control. The mean copy number is 3.9×104 copys/μgDNA in controls, which is much higher than NPC patients (which is 1 7×105 copies/μgDNA). EBV infection is relative with NPC, while the lytic form of EBV maybe more important than its latent form. These results suggest that whole blood EBV DNA may be a valuable tool for molecular diagnosis of NPC.

关 键 词：实时定量PCR方法 检测 鼻咽癌病人 全血EB病毒拷贝数 实验研究 

分 类 号：Q781[生物学—分子生物学]