一种黑豆蚜储存蛋白质的分离纯化及其亚基的性质  

Purification of a Storage Protein from Aphis craccivora Koch and Its Subunit Characterization

作　　者：甘明[1] 丁德诚[1] 周元聪[2] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所蛋白质组学重点实验室,上海200031

出　　处：《生物化学与生物物理学报》2003年第2期189-192,共4页

基　　金：中国科学院院长特别基金资助项目

摘　　要：从黑豆蚜 (AphiscraccivoraKoch)血淋巴中经DE5 2纤维素离子交换层析、SephadexG 15 0凝胶过滤以及在FPLC上QSepharose分离出一种蛋白质。该蛋白质经SDS PAGE测定在非还原和还原条件下分子量均为 6 0kD左右 ,等电聚焦电泳测得其等电点约为 5 .0 ,为糖蛋白。若蚜期间该蛋白质在蚜虫体内积累 ,羽化至成蚜时含量明显减少 ,为蚜虫提供发育所需氨基酸 ,在成蚜期间仍以低浓度存在。根据其分子量和氨基酸组成与含量变化动态应属不完全变态昆虫的一种持续性储存蛋白质。By using of DE52 Cellulose, Sephadex G-150 and FPLC Q Sepharose chromatography, a storage protein (SP) was purified from Aphis craccivora Koch and its subunit was characterized. Its molecular weight was about 60 kD as determined by SDS-PAGE analysis under both reducing and non-reducing conditions, and its pI was about 5.0. It was a glycoprotein. The protein accumulated in larval hemolymph, and decreased when host turned to adult, and could be detected in adults in very low concentration. According to the molecular weight, amino acids composition, and its dynamic alteration of concentrations, the protein should be a persisting storage protein of hemimetabolous insects.

关 键 词：黑豆蚜 储存蛋白质 分离纯化 亚基 性质 昆虫 

分 类 号：Q51[生物学—生物化学]