用发光酶基因(luxAB)标记法研究慢生花生根瘤菌的竞争结瘤能力  

The competitiveness of Bradyrhizobium sp. (Arachis) studied by using luxAB marker gene technique

作　　者：罗明云[1] 张小平[1] 李登煜[1] 陈强[1] 周俊初[2] 

机构地区：[1]四川农业大学 [2]华中农业大学,武汉430000

出　　处：《生态学报》2003年第2期278-283,共6页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目 ( 39970 0 2 9);华中农业大学农业部农业微生物重点实验室开放课题资助项目( AML0 0 1 7)~~

摘　　要：lux AB基因标记是一种新型基因标记技术 ,在很多研究领域都有着良好的应用前景。研究通过三亲本杂交将 lux AB基因成功地向慢生型花生根瘤菌进行了转移 ,并获得了一株带 Lux AB基因标记的菌株Cspr7- 1。对 Cspr7- 1进行性状、标记基因的遗传稳定性检测 ,结果表明 ,Lux AB基因不仅能有效表达 ,而且性状稳定。在无氮水培条件下进行标记菌株与土著根瘤菌的竞争结瘤试验。结果证实 ,Cspr7- 1在植物根系上的占瘤率平均达到 61 .3% ,比土著根瘤菌的竞争结瘤能力强 ,而且 Cspr7- 1在主根上的侵染能力远较侧根上的强 ,平均高出 2 2 .3%～ 39.6%。The luxAB marker gene is useful in microbial ecological studies. One strain Cspr7-1 marked with luxAB gene was obtained triumphantly by thri-patental mating. Detecting the characteristic and stability of Cspr7-1,the results showed that: the luxAB gene not only expressed in Cspr7-1, but also kept stabilized.Using the luxAB marked gene to study competitive ability of Cspr7-1 with soil indigenous rhizobia in N-free solution pot, we found that the Cspr7-1 had higher nitrogen fixing efficiency and stronger competitive ability than that of the soil indigenous bradyrhizobia. Nodule occupancy of Cspr7-1 on peant root was 61.3% on average.The competitive ability of Cspr7-1 was different between main root and lateral root.

关 键 词：发光酶基因 竞争结瘤能力 luxAB基因标记 慢生型花生根瘤菌 

分 类 号：S182[农业科学—农业基础科学]