羟乙基淀粉对油酸型肺损伤大鼠肺毛细血管通透性的影响  

The effect of hydroxyethyl starch on capillary permeability in a rat model of acute lung injury

作　　者：吴镜湘[1] 徐美英[1] 张富军[1] 朱文忠[1] 

机构地区：[1]第二军医大学长海医院麻醉科,上海市200433

出　　处：《中华麻醉学杂志》2003年第3期197-200,共4页Chinese Journal of Anesthesiology

摘　　要：目的 观察6％中分子羟乙基淀粉(贺斯200/0.5)对油酸型肺损伤大鼠肺毛细血管通透性的影响,探讨贺斯对毛细血管渗漏综合征的治疗作用。方法 雄性SD大鼠40只,随机分为4组:I组为对照组,输注平衡液,Ⅱ组为6％贺斯组,Ⅲ组为琥珀明胶(血定安)组,Ⅳ组为右旋糖酐40组;采用大鼠油酸型肺损伤模型(0.06 ml/kg油酸静脉注射),于1.5 h内输注上述分组中的液体10 ml/kg,然后注射0.5％的伊文思蓝1 ml,4 h后处死动物取肺组织,观察肺病理变化,计算肺含水率,用甲酰胺抽提渗漏到肺组织中的伊文思蓝,并测定肺组织中的髓过氧化物酶(MPO)活性及丙二醛(MDA)含量。结果 Ⅱ组肺含水率及伊文思蓝含量显著低于其他各组。病理损害较轻,MPO及MDA水平较低。结论贺斯可降低油酸型肺损伤大鼠肺毛细血管通透性,减轻肺水肿及肺损伤,使MPO及MDA降低,可能与贺斯抑制肺损伤时的中性粒细胞聚集及脂质过氧化有关。Objective To evaluate the effect of 6% hydroxyethyl starch (HES) 200/0.5 on the pulmonary capillary permeability in oleic acid induced acute lung injury (ALI) and investigate the possible mechanism of the therapeutic effect of HES. Methods Forty male SD rats weighing 280-370 g were randomly divided into four groups: group I balanced solution (control); group II 6% HES 200/0.5; group III gelatin and group IV dextran 40. The animals were anesthetized with intraperitoneal 3% pentobarbital 30-40 mg·kg-1 . Right external jugular vein was cannulated. Oleic acid 0.06 ml·kg-1 was given iv.10 ml·kg-1 of the above-mentioned different solutions was infused over 1.5 h in the four groups. Then 0.5% Evan's blue 1ml was injected iv. Four hours later the animals were sacrificed and lungs removed for pathologic examination under microscope, and determination of lung water content [ (wet weight -dry weight)/wet weight × 100% ] . Lung Evan's blue content was determined by formamide extracting method. Lung MDA content and MPO activity were also determined.Results Lung water and Evan's blue contents were significantly lower in group II than those in any other groups. MPO activity and MDA level in group II were lowest among the four groups. Conclusions HES can decrease pulmonary capillary permeability, attentuate pulmonary edema and lung injury, and depress MPO activity and MDA level. The underlying mechanism may be due to its depressant effect on PMN aggregation and lipid peroxidation.

关 键 词：羟乙基淀粉 油酸型肺损伤 大鼠 肺 毛细血管通透性 呼吸窘迫综合征 

分 类 号：R614[医药卫生—麻醉学]