氯胺酮对大鼠大脑皮质和丘脑突触体ATP酶活性的影响  

Effects of ketamine on synaptosomal Na^+ -K^+ -ATPase and Ca^(2+) -ATPase activity of cerebral cortex and thalamus in rats

作　　者：张志龙[1] 李彦东[1] 张来柱[1] 张继跃[1] 段世明[2] 曾因明[2] 

机构地区：[1]济宁医学院附属医院,272000 [2]江苏省麻醉学重点实验室

出　　处：《中华麻醉学杂志》2003年第3期201-203,共3页Chinese Journal of Anesthesiology

基　　金：山东省卫生厅青年基金课题(1999CA2CEAB2)

摘　　要：目的 动态观察氯胺酮对大鼠大脑皮质和丘脑Na+-K+ATP酶和Ca2+-ATP酶活性的影响。方法 SD大鼠32只,随机分为四组:对照组、麻醉组、恢复I组和恢复Ⅱ组。对照组给予腹腔注射生理盐水10 ml·kg-1,10 min后断头;其它三组均为腹腔注射氯胺酮100 mg·kg-1。其中麻醉组在大鼠翻正反射消失后断头;恢复I组在大鼠翻正反射恢复后断头;恢复Ⅱ组在大鼠完全清醒后断头。在生理盐水冰面上分离双侧大脑皮质和丘脑,以分光光度法测大脑皮质和丘脑Na+-K+-ATP酶和Ca2+-ATP酶活性。结果大鼠腹腔注射氯胺酮后,其大脑皮质的Na+-K+-ATP酶和Ca2+-ATP酶活性分别较对照组降低32.8％和26.2％(P<0.05);丘脑的Na+-K+-ATP酶和Ca2+-ATP酶活性也分别较对照组降低31.4％和24.5％(P<0.05);大鼠翻正反射恢复后和动物完全清醒后两种ATP酶活性的变化与对照组相比差异均无统计学意义(P>0.05)。结论 Na+-K+-ATP酶和Ca2+-ATP酶活性的改变在氯胺酮全麻作用机制中可能起重要作用。Objective To investigate the effects of ketamine on Na+ -K+ -ATPase and Ca2+ -ATPase activity Of cerebro cortex and thalamus in rats. Methods Thirty-two SD rats of both sexes weighing 200-300 g were randomly divided into 4 Groups: 1 control group; 2 anesthesia group; 3 recovery group 1 and 4 recovery group II . In control group normal saline 10 ml·kg-1 was administered intraperitoneally (ip) while in the 3 ketamine groups ketamine 100mg·kg-1 was administered ip. The animals were killed by decapitation when righting reflex was lost (group 2) or recovered (group 3) or when the animals were fully awake (group 4). Brain was immediately removed on ice. Bilateral cortex and thalamus were isolated homogenized and centrifuged. Na+ -K+ -ATPase and Ca2+-ATPase activity were assessed by spectrophotometric analysis. Results The Na+-K+-ATPase and Ca2+ -ATPase activity of cerebrocortex decreased by 32.8% and 26.2% and of thalamus decreased by 31.4% and 24.5% respectively in group 2 as compared with those in group 1. The Na+-K+-ATPase and Ca 2+-ATPase activity of the cerebrocortex and thalamus were recovered in group 3 and 4. Conclusions The Na+ -K+ -ATPase and Ca2+ -ATPase may play an important role in the mechanism of ketamine anesthesia.

关 键 词：氯胺酮 大鼠 大脑皮质 丘脑 突触体 ATP酶活性 

分 类 号：R614[医药卫生—麻醉学]