猪MyoG基因的PCR-RFLP多态性分析  

Polymorphism Analysis of Porcine Myogenin Gene by PCR-RFLP

作　　者：林万华[1] 高军[1] 陈克飞[1] 丁能水[1] 艾华水[1] 郭源梅[1] 李琳[1] 黄路生[1] 

机构地区：[1]江西农业大学江西省动物生物技术重点开放实验室

出　　处：《遗传》2003年第1期22-26,共5页Hereditas（Beijing)

基　　金：国家973项目(G2000016100);国家自然科学基金(39860063)资助

摘　　要：以杜洛克、长白、大约克、南昌白、二花脸、梅山猪、玉山黑猪、乐平花猪、金华两头乌及上高两头乌等中外10个猪种共计561头猪为研究材料,采用3对引物(PCR1、PCR2、PCR3)分别扩增猪肌细胞生成素(MyoG)基因的不同区域,扩增产物经限制性核酸内切酶MspⅠ酶切后发现:(1)在PCR1MspⅠ-RFLP位点上,外来品种杜洛克、长白、大约克及培育品种南昌白中极大多数个体表现为AA型,个别为BB型;而6个中国地方猪种除乐平花猪外均以BB型居多。(2)在PCR2MspⅠ-RFLP位点上,6个中国地方猪种除一头玉山黑猪表现为MN型外,其余均为MM型;而外来品种以NN型占大多数,培育品种南昌白更趋向于外来品种。(3)在PCR3MspⅠ-RFLP位点上,所有猪种均可得到扩增产物,但无MspⅠ酶切位点。(4)在梅山猪及与其亲缘关系较近的二花脸猪中,没有发现Soumillion等(1997)报道的梅山猪特异性MspⅠ多态性酶切位点。The polymorphisms of porcine myogenin gene in 561 pigs including Duroc,Landrace,Large Yorkshire,Nanchang white pig,Erhualian,Meishan,Yushan black pig,Leping spotted pig,Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLPs with three different pairs of primers,and the PCR products were digested by MspⅠ.The results showed that most of the Duroc,Landrace,Large Yorkshire,Nanchang white pigs presented as AA genotype,while more animals of the six Chinese local pig breeds except for Leping spotted pig presented as BB genotype at PCR1 MspⅠ-RFLP site.The six Chinese local breeds presented as MM genotype except that one Yushan black pig presented as MN genotype,while more swines of the exotic breeds including Duroc,Landrace,Large Yorkshire presented as NN genotype,and Nanchang white pig appeared to be closer to the exotic breeds at PCR2 MspⅠ-RFLP site.PCR product was obtained in all the swine by PCR3,but the MspⅠrestriction site was not found in the tested pig breeds including Meishan and blood closely related Erhualian pig.

关 键 词：猪 MYOG基因 PCR-RFLP多态性 遗传多态性 肌细胞生成素基因 

分 类 号：Q953[生物学—动物学] S828.2[农业科学—畜牧学]