抗生素G418胁迫条件下转基因水稻种子发芽特性及应用  

The Germinating Characters of the Transgenic Rice Seeds in the Stress Condition of Antibiotic G418 and Their Application in Crop Breeding

作　　者：许明辉[1] 唐祚舜[2] 赵丰萍[1] 田文忠[2] 

机构地区：[1]云南省农业科学院生物技术研究所,昆明650223 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《遗传》2003年第1期45-48,共4页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目(39960039)

摘　　要：在不同浓度的抗生素G418胁迫条件下对抗稻瘟病转基因水稻纯系材料D2-1-2及其受体对照中花9号进行了发芽试验。培养7d的结果表明:不同浓度抗生素处理对两个材料萌动无显著的影响;随抗生素浓度的上升,两个材料发根种子数、种子根长、芽长均受到不同程度的抑制;当抗生素浓度达100mg/L时,转基因材料D2-1-2仍可正常长根(平均1.45cm),但中花9号明显受到抑制(平均0.27cm),二者存在明显差异;根据这一特性,从中花9号和D2-1-2混合群体中筛选出来的长根(大于0.5cm)种子经PCR检测88.46%是转基因个体。The seeds of transgenic rice line D212 and the receptor cultivar Zhonghua No.9 were germinated on the stress condition of the antibiotic G418.The number of taking root seed,the length of root and the length of shoot of two used materials were checked in different concentrations of the antibiotic G418,but the ratio of germinating seed was not affected.At the 100 mg/L level of G418,the transgenic line D212 could take longer root (mean 1.45 cm ) but Zhonghua No.9 very short ( mean 0.27cm ).88.46% of the total longroot (>0.5 cm) seeds selected from the mixing population of D212 and Zhonghua No.9 at the 100 mg/L level of antibiotic G418 were real transgenic ones.

关 键 词：抗生素G418胁迫 水稻种子 发芽特性 应用 转基因水稻 

分 类 号：Q943[生物学—植物学]