采用倍性分析仪鉴定柑橘愈伤组织的遗传变异  

Genetic Variation of Citrus Calli Revealed by the Ploidy Analyser

作　　者：张俊娥[1] 刘继红[1] 邓秀新[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《Acta Genetica Sinica》2003年第2期169-174,共6页

基　　金：国家自然科学基金 (3 983 0 2 60 ) 863计划 (2 0 0 1AA2 4114 1);国际科学基金 (IFS) (D 3 0 0 1 2 )等项目资助~~

摘　　要：使用倍性分析仪 ,对 4 8种培养多年不同基因型的柑橘愈伤组织的细胞DNA含量进行了测定。结果发现 ,除了路比葡萄柚、尾张和金诺橘等 3种愈伤组织变异较小 ,未出现第二个峰以外 ,有 93 8%的基因型的愈伤组织的细胞均出现了DNA含量加倍的细胞。通过DPAC分析软件分析得知 ,凤梨甜橙三倍体、宁波金柑、长沙橘、鲁斯脐橙、国庆 4号和卡特夏橙等 6种愈伤组织的细胞DNA含量还出现了三倍增加及非整倍增加的现象。在待测的 4 8种愈伤组织中 ,DNA含量变异细胞的百分率最大者为凤梨甜橙三倍体 ,达 18 5 1% ;最小者为暗柳橙 ,为 4 70 %。通过邓肯氏新复极差分析得知 ,不同基因型的DNA含量变异细胞的百分率之间存在差异显著性。在相同继代培养基和相同继代周期的条件下 。The cell DNA content of forty eight Citrus calli of different genotype were measured by using the Ploidy Analyser.The results showed that 93 8% out of forty eight Citrus calli had double DNA content except that Ruby?Weizhang and Kinnow had little varied cells and did not show second peak.Moreover,Pineapple?Meiwa kumquat?Changsha?Russ navel?Guoqing No.4 and Carter had DNA varied cells including triploids and aneuploids based on the DPAC software analyse.Among the forty eight Citrus calli,Pineapple had the highest of the DNA varied cells,which was up to 18 57%,Anliucheng had the lowest,4 07%.There existed significant difference in the variation among genotypes by Duncan Analyse.At the same subcultured medium and at the same subcultured period,the effect of cultural duration on the variation of calli was not significant.

关 键 词：倍性分析仪 鉴定 柑橘愈伤组织 遗传变异 

分 类 号：Q943[生物学—植物学]