具有甲状腺素脱碘酶活性的抗体酶的化学修饰  

Chemical Modification of Abzyme with Deiodinase Activity

作　　者：孙立伟[1] 刘立岩[1] 赵大庆[1] 

机构地区：[1]中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春130022

出　　处：《高等学校化学学报》2003年第4期588-590,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重大项目 (批准号 :2 9890 2 80 )

摘　　要：The abzyme with deiodinase activity was modified with diethyl pyrocarbonate(DEPC) and carbodiimide(EDC) respectively. The modified abzymes were dialyzed and their activities were compared with that of the native abzyme. The results indicate that the histidine residue is one of the essential groups in the abzyme, and the modification causes the catalytic activity a loss of 70%. The modification of the residues containing carboxyl group with EDC leads to a loss of 50% activity, indicating that acid amino residues play important roles in the catalytic reaction.The abzyme with deiodinase activity was modified with diethyl pyrocarbonate(DEPC) and carbodiimide(EDC) respectively. The modified abzymes were dialyzed and their activities were compared with that of the native abzyme. The results indicate that the histidine residue is one of the essential groups in the abzyme, and the modification causes the catalytic activity a loss of 70%. The modification of the residues containing carboxyl group with EDC leads to a loss of 50% activity, indicating that acid amino residues play important roles in the catalytic reaction.

关 键 词：甲状腺素脱碘酶 抗体酶 1-乙基-3-13-二甲氨基正丙基)碳化二酰亚胺 酶活性 焦碳酸二乙酯 化学修饰 

分 类 号：Q553[生物学—生物化学]