前体、诱导子及抑制剂对紫杉烷生物合成的促进作用研究  

Improvement of Taxane Biosynthsis in Cell Suspension Cultures of Taxus chinensis by Elicitors,Precursors and Inhibitors

作　　者：汪爱顺[1] 

机构地区：[1]湖北省种子管理站,中国湖北武汉430070

出　　处：《生命科学研究》2003年第1期48-52,共5页Life Science Research

基　　金：国家"九五"科技攻关项目(96 C02 03 01);"863计划"项目(102 12 06 01)

摘　　要：研究了前体、诱导子和抑制剂对中国红豆杉细胞培养生产紫杉烷的促进作用.结果表明,向培养基中加入30mg L3 甲基 2 丁烯 1 醇,2mmol L苯甲酸钠,10mg L矮壮素,100μmol L茉莉酸甲酯,0.1mmol L丝氨酸,可以使紫杉醇含量增加1141.1%;加入0.1mmol L苯甲酸钠,80μmol L茉莉酸甲酯,0.1mmol L丝氨酸可以使2α,5α,10β,14β 四乙酰氧基 紫杉 4(20),11 二烯含量增加134.6%;25mg L矮壮素,100μmol L茉莉酸甲酯,0.5mmol L丝氨酸可以使1β 羟基巴卡亭Ⅰ含量增加95.2%;5mg L3 甲基 2 丁烯 1 醇,10mg L矮壮素,40μmol L茉莉酸甲酯,可以使14β (2 甲基丁酰氧基) 2α,5α,10β 三乙酰氧基 紫杉 4(20),11 二烯的含量增加76.4%.The effects of some elicitors,precursors and inhibitors on production of taxane in cell suspensions of T.chinensis were studied.The results showed that the addition of 30?mg/L 3methyl2buten1ol,2?mmol/L sodium benzoate,10?mg/L chlorocholine chloride,100?μmol/L methyl jasmonate,0.1?mmol/L serine into the cultures increased taxol yields by 1?141.1%;the addition of 0.1?mmol/L sodium benzoate,80?μmol/L methyl jasmonate,0.1?mmol/L serine into the cultures increased 2α,5α,10β,14βtetraacetoxytaxa4(20),11diene yields by 134.6%;the addition of 25?mg/L chlorocholine chloride,100?μmol/L methyl jasmonate,0.5?mmol/L serine into the cultures increased 1βhydroxybaccatin Ⅰyields by 95.2%;the addition of 5?mg/L 3methyl2buten1ol,10?mg/L chlorocholine chloride,40?μmol/L methyl jasmonate into the cultures increased 14β(2methylbutyryloxo)2α,5α,10βtriacetoxxytaxa4(20),11diene yields by 76.4%.

关 键 词：紫杉烷 诱导子 前体 抑制剂 细胞培养 

分 类 号：Q81[生物学—生物工程]