ODQ对清醒大鼠纹状体细胞外基础cGMP水平的影响  

The Effect of ODQ on Basal Extracellular Level of cyclic GMP in Rat Striatum

作　　者：金英[1] 张宝云 于杨[1] 赵艳杰[1] 刘婉珠[1] 刘凤珍[3] 

机构地区：[1]锦州医学院药理学教研室,辽宁锦州121001 [2]锦州市药业集团器化玻有限公司,辽宁锦州121001 [3]锦州医学院物理学教研室,辽宁锦州121001

出　　处：《锦州医学院学报》2003年第1期10-12,共3页Journal of Jinzhou Medical College

摘　　要：目的　应用清醒大鼠脑微透析技术研究可溶性鸟苷酸环化酶抑制剂 1H -〔1,2 ,4〕恶二唑〔4 ,3-a〕喹喔啉 - 1-酮 (ODQ)对大鼠纹状体细胞外cGMP水平的影响。方法　在大鼠纹状体透析探头 ,待大鼠清醒后 2 4h用人造脑脊液进行透析灌流实验。灌流速度为 5 μl·min-1,每 2 0min收集 1次透析液 ,用放免药盒测定cGMP含量。结果　纹状体内局部灌流ODQ10 ,5 0 ,10 0 μmol·L-1,5 μl·min-1可剂量依赖性降低细胞外基础cGMP水平 ,ODQ10 0 μmol-1作用最强、最大 ,可使细胞外基础cGMP水平降低约 5 0 %。结论　本研究实验结果表明在基础状态下纹状体的cGMP约 5 0Objective The nitric oxide-cyclic GMP signalling transduction was studied with a selective inhibitor of solubleguanylyl cyclase,1H(1,2,4)oxadiazolo〔4,3-a〕quinoxalin-1-one(ODQ),during microdialysis of the rat striatum.Methods A microdialysis probe was transversely implanted in rat striatum.After a 24h recovery period,the microdialysis probes were perfused at 5 μl·min -1 with artificial cerebral spinal fluid.Samples were collected every 20 min cGMP was determined by radioimmunoassay.Results Intrastriatal infusion of ODQ 10,50,100μmol·L -1 inhibited,in a concentration-dependent manner,the basal extracellular level of cGMP.The maximl inhibition amounted to about 50%.Conclusion The results suggest that under basic condition,about 50% of the cGMP in the striatum seem to originate from the soluble guanylyl cyclase.It indicated that NO elicits cGMP accumulation in target cells by stimulating soluble guanylyl cyclase in the striatum.

关 键 词：1H-[1 2 4]恶二唑[4 3-a]喹喔啉-1-酮 环鸟苷酸 纹状体 

分 类 号：R977.3[医药卫生—药品]