我国再障患儿血清中人细小病毒B19 NS基因序列测定及变异分析(英文)  

Sequence Analysis and Genetic Variation of NS Gene of Human Parvovirus B19 Isolated from Chinese Children with Acute Aplastic Anemia

作　　者：张国成[1] 苏国平[1] 孙新[1] 许东亮[1] 陈彩平[1] 汤雪晴[1] Biao LI 

机构地区：[1]第四军医大学西京医院儿科,陕西,西安,710032 [2]Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China

出　　处：《中国当代儿科杂志》2003年第1期5-7,11,共4页Chinese Journal of Contemporary Pediatrics

基　　金：National Natural Science Fundation(No.39870021)

摘　　要：目的 探讨B19病毒中国株NS基因的变异。方法 采用聚合酶链反应(PCR)技术,从中国再障患儿血清中扩增人细小病毒B19-XA17,XA18,XA20株的NS基因片段,进行核苷酸序列分析。结果 人细小病毒B19-XA17、XA18、XA20株的NS基因核苷酸序列被测定,测序结果与国外Au株基因核昔的序列比较,有4个核苷酸突变,并引起编码的4个氨基酸发生改变。结论 我国B19病毒NS基因序列与Au株相比有变异。Objective To explore the NS gene structure and mutation of Chinese strains of human Parvovirus B19 (HPV B19). Methods NS genes of R19-XA17, XA18 and XA20 strains from three Chinese children with acute aplastic anemia were amplified by PCR, and then the amplified products were sequenced directly. Results Compared with the sequence of Au strain, four bases of NS genes of B19-XA17, XA18 and XA20 strains changed and their coding amino acids changed also. Conclusions Genetic diversity exists in mucleotide sequences of NS gene of HPV B19 isolated from the serum of Chinese children. [Chin J Contemp Pediatr, 2003, 5(1): 5 - 7]

关 键 词：人细小病毒B19 基因 序列分析 再生障碍性贫血 

分 类 号：R725.5[医药卫生—儿科]