S-100和PTA1在垂体前叶共存的形态学证据  

Morphological evidence of co-localization of PTA1 and S-100 in the anterior pituitary with laser scanning confocal microscopy

作　　者：陈丽华[1] 张新海[1] 张金山[2] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室,陕西西安710032 [2]第四军医大学组织胚胎学教研室,陕西西安710032

出　　处：《医学研究生学报》2003年第2期94-96,T002,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目 (批准号 :3 0 2 70 718)

摘　　要：目的 :观察S 10 0和血小板 T细胞活化抗原 1(PTA1)在正常大鼠垂体前叶细胞的表达和共存。　方法 :免疫荧光双标记结合激光扫描共聚焦显微镜技术进行观察。　结果 :首次证实PTA1免疫反应阳性物质主要位于脑垂体血窦壁 ,与S 10 0免疫反应阳性细胞密切接触 ,并有一定程度的共存关系。　结论Objectives: To determine the expression of platelet and T cell activation antigen 1(PTA1) and S 100 in the normal rat anterior pituitary. Methods: Immunohistochemical method combined with laser scanning confocal microscopy. Results:The intensely staining for PTA1 were found in the endothe lium of the sinuses and were contacted closely with S 100 immunoreactive cells. When double immunolabeling was performed , both PTA1 and S 100 were found to be present in the same cells. PTA1 immunoreactive subtance consists of the minority of S 100 immunoreactive cells. Conclusions: Our results can supply a useful experimental data for the study of the distribution of PTA1 in vivo.

关 键 词：S-100 血小板T-细胞活化抗原1 垂体前叶 激光共聚焦显微镜 

分 类 号：R335.1[医药卫生—人体生理学]