中国汉族人中β_2肾上腺素能受体基因多态性与高血压病的关联性研究  

Association analysis of β_2-adrenergic receptor polymorphisms with hypertension in Chinese

作　　者：朱建[1] 江时森[1] 曹文[2] 张启高[1] 林欣[1] 徐燕丰[2] 

机构地区：[1]南京军区南京总医院心血管内科,江苏南京210002 [2]南京军区南京总医院临床药理科,江苏南京210002

出　　处：《医学研究生学报》2003年第2期123-126,共4页Journal of Medical Postgraduates

摘　　要：目的 :调查 β2 肾上腺素能受体 (β2 AR) 16位和 2 7位基因多态性在中国汉族高血压病人群和正常血压人群中的分布频率 ,并分析β2 受体基因多态性和高血压病是否关联。　方法 :在我院近 2年住院患者中随机选取高血压病 2 19例 ,分为两组 ,发病年龄 <6 0岁者为H1组 ,>6 0岁者为H2组 ;正常血压者 78例 ,也分为两组 ,年龄≥ 4 0岁者为N1组 ,<4 0岁者为N2组。从外周血抽提DNA ,进行聚合酶链反应 (PCR) ,取PCR产物分别用限制性内切酶NcoⅠ和BbvⅠ酶切 ,确定基因型。　结果 :高血压病组 (H组 )中 ,β2 AR 16位点基因型分布与正常血压组 (N组 )比无显著差异 ;而 2 7位点基因型与N组比较 ,比数比 (OR)为 2 .35 ,95 %可信限 (CI)为 1.34～ 4 .15 ,有极显著差异(P <0 .0 1)。等位基因频率也有极显著性差异 (P <0 .0 1)。　结论 :我国汉族人群中 β2 AR基因 2Objectives: To analyze the association between β 2 adrenergic receptor genetic polymorphisms and hypertension in Chinese. Methods: A case control study was conducted in 219 hypertensive and 78 normotensive subjects. Genomic DNA was prepared from peripheral blood. The PCR product which includes the region of the β 2 AR 16 and the β 2 AR 27 polymorphism were digested with NcoⅠ and BbvⅠ respectively to identify the genotype. Results:There was no significant difference in the frequency of Gly/Gly genotype in the hypertensive subjects compared with the normotensive subjects. There was a significant difference in the frequency of Gln/Gln genotype in the hypertensive subject compared with the normotensive subjects(OR:2.35,95% CI:1.34～4.15, P <0.01). Conclusions:These results suggest that β 2 AR 27 locus genetic polymorphism is associated with essential hypertension in Chinese.

关 键 词：高血压病 Β2肾上腺素能受体 基因多态性 

分 类 号：R544.1[医药卫生—心血管疾病]