Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray  

作　　者：FANGLIN SHOULINGXU 等 

机构地区：[1]NationalaboratoryofPlantMolecularGenetics,InstituteofPlantPhysiologyandEcology,ShanghaiInstitutesforBiologicalScience（SIBS）,ChineseAcademyofSciences andPartnerGroupofMax－Planck－In [2]NationalaboratoryofP

出　　处：《Cell Research》2003年第1期59-68,共10页细胞研究（英文版）

基　　金：Researches were supported by "the State Key Project of Basic Research, G1999011604"; "Key Project of Knowledge Innovation, CAS", "the National Natural Science Foundation of China, No.30070073" ; "National Sciences Foundation of Pan-Deng". We thank Prof.

摘　　要：Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processesincluding seed maturation, germination and response to environmental factors. With the purpose to detectthe possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes throughcDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least onetime, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealedthat the putative encoded proteins were involved in different possible processes, including transcription,metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones werefound to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signaltransduction or metabolism was confirmed by reverse transcription PCR. In addition, some clones werefurther shown to be regulated by other plant growth regulators including auxin and brassinosteroid, which,however, indicated the complicated interactions of plant hormones. Possible signal transduction pathwaysinvolved in ABA were discussed.

关 键 词：Oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion 

分 类 号：Q946.885.6[生物学—植物学] Q943