紫外线照射对牛孤雌激活卵母细胞和体细胞核移植胚胎体外发育的影响  

Effects of ultraviolet irradiation on in vitro development of parthenogeneticand somatic nuclear transferred embryos in bovine

作　　者：李雪峰[1] 安志兴[2] 李向臣[2] 张涌[2] 

机构地区：[1]华南师范大学生命科学学院,广东广州510631 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国兽医科技》2003年第3期20-24,共5页Chinese Journal of Veterinary Science and Technology

基　　金：国家高技术研究发展资助项目(2 0 0 1AA2 1 30 81 )

摘　　要：分析了紫外线 (ultraviolet,UV)照射对牛孤雌激活卵母细胞和体细胞核移植胚胎卵裂和体外发育的影响。结果发现 ,经UV照射 2 0s和 40s的卵母细胞孤雌激活后的卵裂率和囊胚发育率都极显著低于UV照射 0s和 1 0s的卵母细胞 (P <0 .0 1 ) ,表明UV照射卵母细胞超过 2 0s降低了孤雌激活胚胎的卵裂和体外发育潜力。核移植中UV照射卵母细胞 2 0s后 ,重组胚的卵裂率极显著低于卵母细胞照射 0s和 1 0s时所构建的重组胚 (P <0 .0 1 ) ,但囊胚发育率无显著差异 (P>0 .0 5 ) ;而UV照射卵母细胞 40s时 ,所构建的重组胚的卵裂率和囊胚发育率都明显低于其他各组 ,表明超过 2 0s的UV照射显著降低了重组胚的卵裂和体外发育潜力。研究结果表明 ,核移植中用UV指导卵母细胞去核时UV照射时间应控制在 2 0s以内 ,UV照射 1Effects of ultraviolet(UV) irradiation on the cleavage and in vitro development of parthenogenic and somatic cloned embryos in bovine were investigated. The results showedthat the cleavage and blastocyst development rates of parthenogenic embryos derived from oocytes irradiated with UV for 20s and 40s were significantly lower than those of oocytes irradiated with UV for 0s and 10s(P<0.01). It indicated that irradiating oocytes with UV for longer than 20s decreased the developmental potential of parthenogenic embryos significantly. In cloning research, although the cleavage rate of reconstructed embryos derived from the enucleated oocytes suffered from UV irradiation for 20s is significantly lower than that of enucleated oocytes suffered from UV irradiation for 0s and 10s (P<0.01), the blastocyst development rate had no significant difference among them. The cleavage rate and blastocyst development rate of reconstructed embryos derived from enucleated oocytes suffered from UV irradiation for40s were lower than other groups significantly. The results indicated that irradiating enucleated oocytes with UV for longer than 20s decreased the cleavage and in vitro developmental potential of reconstructed embryos. In cloning research, the UV irradiation should be shorter than 20s when the Hoechst dye and UV irradiation were used in the enucleation, while 10s UV irradiation on enucleated oocytes haven't any harmful effect on the development of reconstructed embryos.

关 键 词：紫外线照射 牛 卵母细胞 体细胞核 体外发育 孤雌生殖 UV照射 胚胎移植 

分 类 号：S823[农业科学—畜牧学]