2型糖尿病视网膜病与血小板粘合素α_2β_1基因多态性的相关性  

Association Between the Gene Polymorphism in Plateletα_2β_1 Integrin and Type 2 Diabetes with Retinopathy

作　　者：张军霞[1] 徐焱成[1] 朱宜莲[1] 

机构地区：[1]武汉大学中南医院内分泌科,武汉430071

出　　处：《武汉大学学报（医学版）》2003年第2期124-126,共3页Medical Journal of Wuhan University

摘　　要：目的 :探讨血小板粘合素α2 β1 分子α亚单位第 7内含子BglⅡ限制性片段长度多态性与 2型糖尿病视网膜病 (DR)的关系。方法 :运用聚合酶链反应 限制性片段长度多态性技术 (PCR RFLP)检测 12 5例 2型糖尿病 (DM)患者 [DR(+)组 5 6例 ,DR(- )组 6 9例 ]及 4 5例正常对照组的基因型。结果 :①DR +组BglⅡ (+ +)基因型频率及BglⅡ (+)等位基因频率明显高于DR -组 (P <0 .0 5 )及正常对照组 (P <0 .0 1) ,BglⅡ (+)等位基因与DR明显相关(OR =1.88,95 %CI,1.14～ 3.10 ) ;②DR -组与正常对照组BglⅡ (+ +)基因型频率及BglⅡ (+)等位基因频率无显著性差异 (P >0 .0 5 ) ,单纯型DR与增殖型DR基因型及等位基因频率分布差异无显著性 (P >0 .0 5 )。结论 :α2 β1 分子α亚单位BglⅡ限制性片段长度多态性与DR相关 ,与DM无关。BglⅡ (+)等位基因可能是 2型DM合并视网膜病的危险因子。Objective: To evaluate the role of BglⅡ restriction fragment length polymorphism within intron 7 of the α sununit of platelet α 2β 1 integrin in Chinese patients with diabetic retinopathy 1(DR). Methods: 125 patients(56 patients with retinopathy, DR+group; and 69 patients without retinopathy, DR-group) and 45 normal subjects(controls) are recruited in the study. BglⅡ polymorphism is determinded by PCR RFLP. Results:①The frequencies of the BglⅡ(+/+) genotype and BglⅡ(+) allele in DR+group are significantly higher than that in DR-group(P<0.05=and controls(P<0.01);BglⅡ(+) allele is associated with DR(OR=1.88,95%CI,1.14～3.10), but not DM; ②The genotype frequency for BglⅡ(+/+) and the allele frequency for BglⅡ(+) are not significantly different between DR group and controls (P>0.05). For simple DR group and proliferative DR group, genotype distributions do not differ significantly(P>0.05). Conclusion: BglⅡpolymorphism of theαsubunit within platelet α 2β 1 integrin play a role in the pathogenesis of DR in Chinese, but not DM. BglⅡ(+) allele may contribute to DR.

关 键 词：糖尿病视网膜病 血小板粘合素 α2β1分子 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]