DNA提取的应用与相关技术分析  

Application of DNA Extraction and Analysis of Its Influence Factors

作　　者：董明[1] 宫月华[1] 王兰[1] 袁媛[1] 

机构地区：[1]中国医科大学第一临床学院肿瘤研究所,沈阳110001

出　　处：《遗传》2003年第2期205-207,共3页Hereditas（Beijing)

基　　金：国家自然科学基金项目资助(30171054)

摘　　要：为了分析影响提取DNA的有关因素,采用标准酚—氯仿抽提法和蛋白酶消化法提取DNA。结果表明,平均每mL全血可提取200～300μgDNA;新鲜组织及OCT包埋冰冻组织提取DNA,平均每0.2g组织可获得200～300μgDNA;直接将石蜡标本提取物制作模版,PCR扩增良好。从4种组织中均获得较为纯净的DNA;OCT对组织DNA无不良影响。To explore influence factors of DNA extraction,the protease and phenol-chloroform method was used to extract DNA in whole blood,fresh tissues,frozen tissues embedding with OCT and tissues embedding in paraffin.It results that 200～300μg DNA was extracted from 1ml whole blood or 0.2g fresh tissues or frozen tissue embedding with OCT.DNA extracted from paraffin specimen can be directly used in PCR amplification.Purity DNA can be extracted from four kinds of different tissues.OCT hasn't harmful effect on tissue DNA extraction.

关 键 词：DNA提取 血液 新鲜组织 石蜡标本 冰冻组织 

分 类 号：Q523[生物学—生物化学]