从水稻种胚中提取RNA的新方法  

A Novel Method of Extracting Total RNA from Rice Embryo Samples

作　　者：沈文飚[1] 汪仁[1] 王益华[1] 郑天清[1] 万建民[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室南京农业大学水稻研究所,南京卫岗210095

出　　处：《遗传》2003年第2期208-210,共3页Hereditas（Beijing)

基　　金：科技部转基因专项(J00-A006-1);国家自然科学基金资助(No.30000013和No.30170570)项目

摘　　要：介绍了一种全新、简单的提取水稻种胚RNA的CTAB-LiCl提取法,可在不用液氮的室温下有效地排除剥取的种胚中大量带有的多糖和本身脂质的干扰,所得RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性,并可进一步满足RT-PCR和Northern印迹的实验需要,适用于富含多糖和脂质的植物组织RNA的提取。A novel and simple CTAB-LiCl-based extraction method for high-quality and total RNA of rice embryo samples is developed.This method can efficiently eliminate the interference of polysaccharide and lipids rich in rice(Oryza sativa L.)embryo obtained under room temperature without using liquid nitrogen.The results of ultraviolet spectrophotometer and agarose gel electrophoresis analysis show that the obtained RNA has no obvious degradation and a good purity sufficient for further RT-PCR and RNA gel blotting.Therefore,it is also especially useful for the RNA extraction of plant material plenty of polysaccharide and lipids.

关 键 词：水稻种胚 RNA CTAB-LiCl提取法 多糖 脂质 

分 类 号：Q943.2[生物学—植物学]