骨髓基质成骨细胞与煅烧骨联合培养的实验研究  

Experimental study of marrow stromal osteoMasts culture with caldned bone caldam

作　　者：陆伟 陶凯 毛天球 杨维东 马立 赵丽君 

机构地区：[1]解放军第四军医大学口腔医院颌面外科,陕西省西安市710032

出　　处：《中国临床康复》2003年第2期214-215,T002,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家"973"项目资助课题(G1999054308)

摘　　要：目的:探索煅烧骨(calcined bone calcium,CBC)作骨组织工程支架材料的可行性。方法:将CBC分纤维粘连蛋白修饰组和单纯培养液修饰组,分别与骨髓基质成骨细胞(marrow stromal osteoblast,MSO)于体外联合培养,进行扫描电镜观察和碱性磷酸酶活性检测,了解细胞在材料中的粘附、生长、增殖、分泌及材料与细胞的相互作用情况。结果:经系统处理过的CBC具有类似原骨的三维结构。体外培养12h细胞已贴附于CBC支架,复合培养7d,分布在支架上的细胞迅速分化增殖,分泌细胞外基质和矿化结节。碱性磷酸酶测定7d组的活性显著高于12h组活性(P<0.01)。两组之间扫描电镜观察和碱性磷酸酶测定均未见明显差异(P>0.05)。结论:CBC生物相容性好,不需作修饰即能促进成骨细胞的粘附、增殖、分泌活动,是骨组织工程的理想支架材料。AIM: To investigate the feasibility of calcined bone calcium (CBC) as scaffold material in bone tissue engineering. METHODS: The marrow stromal osteoblasts(MSO) were seeded on CBC treated with fibronectin and culture medium only. Then the cells/CBC composites were cultured in vitro. The composites were observed for the processs of cell growth, matrix formation and the interaction between cells and scaffolds at 12,24 hours and 7 days under electronic scanning microscope and examined ALP activity by immunohisto-chemistry. RESULTS: 12 hours after culture, the cells had already adhered to the scaffold. 7 days later the cells differentiated and proliferated in the network of CBC. Extracellular matrix was secreted and calcium nodes were formed among MSO. ALP activity of the 7 days' group was significantly higher than the 12 hours' one (P < 0.01) . But the results between the treated with fibronectin group and culture medium only group were not apparently different (P > 0. 05). CONCLUTION: The results show that CBC has great cellar biocompatibility. It can promote cell adhesion, proliferation and secretory while needn' t modification . It is an ideal scaffold material for bone tissue engineering.

关 键 词：骨髓基质成骨细胞 锻烧骨 骨组织工程支架材料 体外培养 

分 类 号：R68[医药卫生—骨科学] R318.08[医药卫生—外科学]