鸡新城疫病毒B_(95)株的RT-PCR检测方法研究  

Studies on detection of NDV strain B_(95) by RT-PCR

作　　者：申之义[1] 苗儒 王俊平 焦静波[3] 信洪一[1] 李平安[1] 

机构地区：[1]内蒙古农业大学,呼和浩特市010018 [2]内蒙古动物防疫监督检验所,010051 [3]包头市动物防疫监督检验所,014040

出　　处：《中国动物检疫》2003年第4期23-25,共3页China Animal Health Inspection

摘　　要：本试验设计了一对B95 株的特异引物NDV -P1/P2,探讨了对其进行RT-PCR检测的可行性。结果证明 ,用该引物可以扩增出一条310bp的特异核酸带 ,敏感性为0.0043ng/ul。A pair of specific primers used in RT-PCR were designed according to the sequences of NDV B95 strain. The RT-PCR detection for NDV strain B95 was studied. Result showed that it was specific to strain B95 and the sensitivity is 0.0043ng/ul.

关 键 词：鸡 新城疫病毒 B95株 RT-PCR检测方法 

分 类 号：S858.31[农业科学—临床兽医学]