家蝇(Musca domestica VicinaL.)头部乙酰胆碱酯酶检测农药残留最佳条件研究  

Studies on the Optimum Conditions of Detecting PesticidesResidue by Using Acety Lcholinesterase inHousefly (Musca domestica Vicina L.) Head

作　　者：董杰[1] 王开运[1] 姜兴印[1] 仪美芹[1] 

机构地区：[1]山东农业大学植物保护学院农药系,山东泰安271018

出　　处：《农药学学报》2003年第1期93-96,共4页Chinese Journal of Pesticide Science

基　　金：国家"十五"科技攻关项目 (2 0 0 2 BA5 16 A12 )

摘　　要：系统研究了敏感家蝇 Musca domestica Vicina L .羽化后不同天数其头部乙酰胆碱酯酶(ACh E)的比活力、敏感度以及酶源蛋白质含量的变化 ;缓冲液 p H值对家蝇头部 ACh E比活力的影响。结果表明 :家蝇初羽化至羽化后第 15 d,其头部 ACh E的比活力逐渐升高 [0 .4 483± 0 .0 10 4～ 0 .776 9± 0 .0 189μmol/ (mg· min) ],而酶源蛋白质的含量逐渐下降 (187.0 2 2 2± 9.0 2 13～83.0 2 2 2± 7.1111μg/头 )。羽化后 2～ 4 d,其头部 ACh E的敏感度最高 ;初羽化和羽化后第 15 d,敌敌畏的 I50 分别是羽化后第 3d的 1.4倍和 2 .1倍。在一定的 p H值范围内 (6 .6～ 8.0 ) ,家蝇头部ACh E的比活力随缓冲液 p H值的升高而增大。本研究为利用家蝇头部 AChThe changes of the activity and susceptibility of acetylcholinesterase (AChE) and the changes of the content of protein of susceptible housefly ( Musca domestica Vicina L.) after emergence were investigated systemically, and the effects of different pH values on the activity of AChE were also studied. The results showed that, the specific activity of AChE in the head increased gradually[0.448 3±0.010 4～0.776 9± 0.018 9 μmol/(mg·min)] from the first day to the fifteenth day after emergence, while the content of protein decreased gradually (187.022 2±9.021 3～83.022 2±7.111 1 μg/head). The susceptibility of AChE in the head increased to highest level from the second day to the fourth day after emergence, and the I 50 of dichlorvos of the first day and the fifteenth day after emergence was 1.4 times and 2.1 times compared to that of the third day,respectively. The specific activity of AChE in the head increased with the increasing of the pH value within some scope. These results offered an optmum conditions for detecting phosphorus pesticide residue using AChE from housefly head.

关 键 词：家蝇 乙酰胆碱酯酶 比活力 敌敌畏 敏感度 

分 类 号：Q965.9[生物学—昆虫学]