来源于肾上腺髓质素原的不同肽段对大鼠主动脉一氧化氮生成的影响  

Effects of peptides originated from the proadrenomedullin on the production of nitric oxide in rat aorta

作　　者：董献红[1] 李菊香[2] 钟光珍[2] 任永生[2] 吴胜英[2] 唐朝枢[2] 

机构地区：[1]河南新乡医学院生理教研室 [2]北京大学基础医学院生理学和病理生理学系,北京100083

出　　处：《北京大学学报（医学版）》2003年第2期146-149,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金 (30 170 34 7)~~

摘　　要：目的 :观察肾上腺髓质素 (ADM)对大鼠主动脉一氧化氮 (NO)生成的作用 ,以及肾上腺髓质素原N端 2 0肽 (PAMP)和肾上腺升压素 (ADT)对ADM这一作用的影响。方法 :ADM ,PAMP和ADT孵育离体大鼠主动脉 2h后 ,测定孵育液中亚硝酸盐的含量和所孵育组织中一氧化氮合酶 (NOS)的活性 ,其中亚硝酸盐的含量反映NO的生成量。结果 :ADM(10 -9～ 10 -7mol·L-1)浓度依赖地刺激大鼠主动脉组织亚硝酸盐的生成和NOS的活性。 10 -8mol·L-1的ADM孵育血管后 ,每毫克蛋白亚硝酸盐的生成量为 (0 .2 82± 0 .0 4 6) μmol,NOS活性为 (0 .3 2 3± 0 .0 5 6)pmol·min-1,显著高于对照组 [每毫克蛋白 (0 .173± 0 .0 2 6) μmol和 (0 .110± 0 .0 2 8)pmol·min-1,P <0 .0 1]。此浓度的ADM与相同浓度的PAMP或ADT共同孵育血管后 ,每毫克蛋白亚硝酸盐的生成量为 (0 .2 0 4± 0 .0 4 9)或(0 .15 0± 0 .0 3 6) μmol,NOS活性为 (0 .178± 0 .0 2 3 )或 (0 .12 3± 0 .0 3 1) pmol·min-1;ADM ,PAMP和ADT三者共同孵育 ,每毫克蛋白亚硝酸盐的生成量为 (0 .162± 0 .0 2 9) μmol,NOS的活性为 (0 .110± 0 .0 2 4 ) pmol·min-1,均显著低于ADM (10 -8mol·L-1)单独孵育组 (P <0 .0 1)。Objective: To observe the effect of adrenomedullin (ADM) on the production of nitric oxide (NO) in rat aorta and the effect of Proadrenomedullin N terminal 20 peptide (PAMP) and adrenotensin (ADT) on the ADM induced NO production. Methods: Isolated aortic tissues were exposed to ADM, PAMP and ADT for 2 h. The NO production, indicated by nitrite content in the incubated media, and the nitric oxide synthase (NOS) activity in the incubated tissues were assayed. Results: Nitrite productions and NOS activities of the aortic tissues were significantly increased by ADM in a concentration dependent manner. The nitrite production and NOS activity of the aortic tissues stimulated by ADM (10 -8 mol·L -1 ) incubation were (0.282±0.046) μmol per mg protein and (0.323±0.056) pmol ·min -1 per mg protein,respectively, which were greater than those of the control (0.173± 0.026) μmol per mg protein and (0.110±0.028) pmol·min -1 per mg protein ( P < 0.01), respectively. The nitrite production and NOS activity were (0.204±0.049) μmol per mg protein and(0.178±0.023) pmol·min -1 per mg protein when the tissues were treated with ADM (10 -8 mol·L -1 ) and PAMP (10 -8 mol·L -1 ) in combination, and were (0.150±0.036) μmol per mg protein and (0.123±0.031) pmol·min -1 per mg protein when ADM (10 -8 mol·L -1 ) and ADT (10 -8 mol·L -1 ) were used in combination, which were significantly less than those in ADM (10 -8 mol·L -1 ) group. After incubation of the aortic tissues with the same concentrations(10 -8 mol·L -1 ) of ADM, PAMP and ADT in combination, the nitrite production and NOS activity were (0.162± 0.029) μmol per mg protein and (0.110±0.024) pmol·min -1 per mg protein, which were also greatly reduced as compared with those of the ADM group (10 -8 mol·L -1 , P <0.01). However, neither PAMP nor ADT had effect on the production of nitrite and NOS activity in the aortic tissues. Concl

关 键 词：肾上腺髓质素 肾上腺髓质素原N端20肽 肾上腺升压素 一氧化氮 一氧化氮合酶 主动脉 

分 类 号：R331.3[医药卫生—人体生理学]