微生物群落结构探针杂交评价不同培养基从活性污泥分离优势菌群的能力  

Capacity Evaluation of Different Media for Isolating Predominant Phenol-degrading Bacteria from Activated Sludge with Community Structure-specific DNA Probes

作　　者：高平平[1] 赵立平[1] 等 

机构地区：[1]上海交通大学生命科学技术学院，上海200240

出　　处：《微生物学报》2003年第2期264-270,共7页Acta Microbiologica Sinica

基　　金：863计划资助项目 (SZ 0 3 0 1 0 4;2 0 0 1AA2 1 41 3 1 )~~

摘　　要：用ERIC PCR (EnterobacterialRepetitiveIntergenicConsensus PCR)、苯酚羟化酶大亚基基因 (LmPHs)扩增和群落结构探针分子杂交检测技术对LB、dCGY、MP和FWM 4种培养基从焦化废水处理厂 2个曝气池活性污泥中分离优势功能菌群的能力进行了比较研究。LmPHs扩增显示 7种回收菌群中均有以多亚基苯酚羟化酶为代谢途径的苯酚降解菌存在。用代表苯酚降解高峰期活性污泥优势菌组成的总DNA的ERIC PCR产物经地高辛标记作为群落结构的混合探针M1和M8,对 8种回收菌群的ERIC PCR指纹图谱进行杂交检测 ,不同培养基回收优势菌的能力不同 ,以废水为基础的FWM培养基从活性污泥中回收到的优势菌种群最多( 30 8%～ 42 9% )。The capabilities of LB, dCGY, MP and FWM media for the recovery of predominant phenol degrading bacteria in activated sludge samples from the aeration tanks of a two stage system for coking wastewater treatment were evaluated with ERIC PCR(Enterobacterial Repetitive Intergenic Consensus PCR)profiling, PCR detection of the largest subunit of phenol hydroxylase (LmPHs) and hybridization with community structural DNA probes. The specific amplification of LmPHs revealed that seven out of eight recovered mixed populations employed the multi component phenol hydroxylase to degrade phenol. ERIC PCR products amplified from total DNA of activated sludge samples with high phenol removal efficiency were DIG labeled as mixed probes reflecting composition of predominant bacteria in the sludge samples to hybridize with ERIC PCR fingerprints amplified from total DNA of pooled colonies recovered from different isolation media as a way to evaluate the media's capacity for recovering predominant bacteria from activated sludge systems. It was demonstrated that the feeding water based medium FWM recovered the highest proportion (30 8%～42 9%) of predominant populations from activated sludge samples in the first aeration tank. A new strategy has been proposed for evaluating capacity of different media in terms recovery of predominant populations from activated sludge systems.

关 键 词：分离培养基 活性污泥 苯酚降解菌 ERIC-PCR 群落结构 微生物群落 

分 类 号：Q93-3[生物学—微生物学]