细胞因子在银屑病非皮损区成纤维细胞中的表达  

Cytokine Gene Expression on Fibroblasts from Psoriatic Uninvolved Skin

作　　者：陆毅[1] 冯信忠[1] Corrine Lebreton Bernard Coulomb Louis Dubertret 

机构地区：[1]第二医科大学附属瑞金医院皮肤科,上海200025 [2]法国巴黎圣路易医院皮肤研究所

出　　处：《中华皮肤科杂志》2003年第3期133-135,共3页Chinese Journal of Dermatology

基　　金：Rene Tourane基金资助

摘　　要：目的探讨成纤维细胞分泌的细胞因子在银屑病发病中的作用。方法分别取寻常性银屑病患者非皮损区及正常人皮肤的成纤维细胞进行培养,并建立体外皮肤模型。提取其中的成纤维细胞中的RNA用于cDNA微阵列法检测,比较正常人和银屑病患者非皮损区成纤维细胞在细胞因子基因表达水平的差异。结果在268个细胞因子及其受体相对应的基因中,与正常人组相比,银屑病组的成纤维细胞表达上升的有24个,下降的有3个。结论银屑病患者非皮损区的成纤维细胞较正常人的成纤维细胞具有更强的促表皮细胞增殖作用,其细胞因子的表达提示银屑病患者非皮损区具有潜在的向银屑病皮损发展演变的可能。Objective To study the cytokines secreted by fibroblasts in the pathogenesis of psoriasis.Methods The healthy or psoriatic fibroblasts were co-cultured with keratinocytes in skin equivalents for15days.Changes in the expression pattern of268selected genes related to cytokines and their receptors were determined by comparing mRNA levels of fibroblasts under different culture conditions.Results Sixty-eight of268genes were found to be expressed on the fibroblasts.Compared with normal controls,a total of27genes was found to be differentially expressed at RNA level by a factor of twofold or greater,among which24genes were up-regulated,whereas3genes were down-regulated in psoriatic fibroblasts.These differentially expressed genes encoded interleukins,interferons,growth factors,chemokines,protein kinase receptors and chemokine receptors.Conclusions These results suggest that fibroblasts from psoriatic uninvolved skin strongly express cytokines which closely relate to the pathogenesis of psoriasis.It is indicated that dermal fibroblasts may play a potentially role in promoting keratinocyte proliferation and leading to the formation of psoriatic lesion.

关 键 词：银屑病 成纤维细胞 cDNA微阵列技术 细胞因子 基因表达 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]