小麦耐盐突变体盐胁迫下SIR73基因片段的分离和鉴定  

Isolation and Characterization of SIR73 Gene Fragment in salt resistant mutant of Wheat Involved in Salt Stress

作　　者：陈桂平[1] 马闻师[1] 黄占景[1] 沈银柱[1] 

机构地区：[1]河北师范大学生命科学院

出　　处：《遗传》2003年第2期173-176,共4页Hereditas（Beijing)

基　　金：国家自然科学基金(30070471);河北省自然科学基金(301103)

摘　　要：采用cDNA-AFLP(cDNA-amplifiedfragmentlengthpolymorphism)技术,分析"一粒传"后代小麦耐盐突变体中,耐盐性不同的两种小麦品系盐胁迫前后的基因表达差异,得到大量与耐盐相关的基因片段。其中73号片段与人类转录因子基因有一定的同源性(相应部分的同源性32%),命名为SIR73(saltinducibleinresistance)。North ern杂交证明它是受盐胁迫诱导表达的基因,并且在SR品系中其诱导表达更为明显,推测此片段可能在小麦盐胁迫下基因表达调控方面起作用。cDNA-AFLP (amplified fragment length polymorphism) is used to isolate genes differentially expressed in two wheat lines with the different resistantace to NaCl derived from a single seed.A lot of cDNA fragments related to salt tolerance are obtained.Of with the number 73 cDNA fragment encodes for a transcription factors with an 32% similarity to human transcription factors in the relative amino acid which is named SIR73.Northern analysis confirms that SIR73 is strongly induced by NaCl stress and the expression in SR is more strongerly induced than in SS.SIR73 may be involved in the regulation of gene expression in salt stress in wheat.

关 键 词：小麦 盐胁迫 耐盐突变体 CDNA-AFLP技术 转录因子 

分 类 号：S512.1[农业科学—作物学]