一株异养硝化细菌的分离及系统发育分析  

Isolation, Identification and Phylogenetic Analysis of a Heterotrophic Nitrifier

作　　者：张光亚[1] 陈美慈[2] 韩如旸[2] 闵航[2] 

机构地区：[1]中国科学院南京土壤研究所土壤圈物质循环开放研究实验室,南京210008 [2]浙江大学生命科学学院生物科学系,杭州310029

出　　处：《微生物学报》2003年第2期156-161,共6页Acta Microbiologica Sinica

基　　金：浙江省自然科学基金 (3 992 1 4);中国科学院南京土壤研究所土壤圈物质循环开放研究实验室基金资助项目

摘　　要：从大棚土壤中分离到一株异养型硝化细菌 ,命名为菌株HN ,分离菌株为革兰氏染色阳性 ,球状或杆状。菌落颜色为橙红色。该菌株能以乙酰胺为唯一碳源和氮源进行氨化作用和硝化作用并产生亚硝酸。以硝酸钠为氮源时能进行反硝化作用。部分长度的 1 6SrDNA序列分析表明 ,分离菌株HN与Rhodococcusruber具有 99%相似性。并用PHYLIPS程序将该菌株与报道的相关硝化细菌进行系统发育进化分析。本文首次报道Rhodococcussp .A heterotrophic nitrifier, named strain HN, was isolated from the greenhouse soil. The cells of isolates were Gram positive, rod or coccus. The colonies of the strain were orange red. Strain HN could ammonize organic nitrogen compounds and nitrify ammonia itself when it grew on acetamide companying the formation of ammonia and nitrite. It also could denitrify nitrate companying the formation of nitrite. The phylogentic analysis based on 16S rRNA suggested strain HN was the closest relative of Rhococcus ruber with 99% sequence identity. The phylogenetic analysis of strain HN was performed by PHYLIPS and the phylogenetic tree of strain HN and the neighbouring nitrifiers was given. This paper first reported Rhococcus sp. strain HN is a heterotrophic nitrifier.

关 键 词：异养硝化细菌 16SRDNA序列 系统发育 

分 类 号：Q939.9[生物学—微生物学]