Structure and Expression Analyses of a Gene Encoding Fructose-6-Phosphate, 2-Kinase/Fructose-2,6-Bisphosphatase from Maize  

玉米果糖-6-磷酸,2-激酶/果糖-2,6-二磷酸酶基因的结构与表达分析(英文)

作　　者：王东[1] 杜喜玲[2] 张红生[1] 钱晓茵[2] 杨金水[2] 翟虎渠[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京210095 [2]复旦大学生命科学院遗传学研究所,上海200433

出　　处：《Acta Botanica Sinica》2003年第4期466-471,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：教育部博士点基金 ( 2 0 0 10 30 70 0 3)~~

摘　　要：A full-length cDNA encoding fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from maize (Zea mays L.) was cloned by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and designated as mF2KP. The encoded protein is composed of two regions. Its COOH-terminal region is catalytic region and homologous to the enzymes from other eukaryotes; and its NH 2-terminal region is common and special region only in plant. A truncated fragment of mF2KP covering integrated catalytic region was expressed in Escherichia coli. The fusion protein had the activities of fructose-6-phosphate, 2-kinase as well as fructose-2,6-bisphosphatase. Northern blot showed that the transcript level of mF2KP in seedlings initiated from strong-vigor seeds is lower than that from weak-vigor seeds.通过RT_PCR ,结合RACE技术 ,得到了玉米 (ZeamaysL .)果糖_6_磷酸 ,2_激酶 /果糖_2 ,6_二磷酸酶的全长cDNA克隆 ,命名为mF2KP。氨基酸序列同源性比较发现 ,mF2KP蛋白可以分为两个部分 :C端包含高度保守的催化功能区 ,N端为植物中特有的多肽。将mF2KP基因中一段包含完整催化功能区的片段在大肠杆菌 (Escherichiacoli)中表达 ,融合蛋白具有果糖_6_磷酸 ,2_激酶 /果糖_2 ,6_二磷酸酶活性。Northern杂交证明在种子活力不同的幼苗中 ,mF2KP的转录水平存在明显差异。种子活力越高 ,幼苗中mF2KP的转录水平越低。

关 键 词：maize fructose-2 6-bisphosphate fructose-6-phosphate 2-kinase/fructose-2 6-bisphosphatase seed vigor 

分 类 号：S513[农业科学—作物学]