增强组织工程支架:纤维蛋白胶稳定性的初步研究  

The initial study to strengthen tissue engineering stand fibrin glue stability

作　　者：张力[1] 李奇[1] 唐银丽[2] 吴广森[1] 田京[1] 

机构地区：[1]解放军第一军医大学珠江医院脊柱骨病科,广东省广州市510282 [2]解放军第一军医大学珠江医院消化内科,广东省广州市510282

出　　处：《中国临床康复》2003年第2期218-219,T002,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省自然科学基金资助项目(2002C31303)

摘　　要：目的:研究抑肽酶和氨甲环酸对于减缓纤维蛋白胶支架降解速度的作用。方法:将抑肽酶和氨甲环酸加入纤维蛋白胶中,构建细胞标准支架复合物和细胞增强支架复合物,体外培养,观察软骨细胞在支架中的繁殖情况、形态学、超微结构检测以及载体降解情况。结果:两组复合物体外培养一二周后,其质量有显著性差异;两组复合物的细胞生长曲线无明显差别;软骨细胞在纤维蛋白胶支架中立体培养可分泌胞外基质。结论:在纤维蛋白胶中加入抑肽酶和氨甲环酸,可显著的减缓纤维蛋白胶的降解速度,并且对软骨细胞的繁殖、表型的维持、基质的分泌无不良影响。AIM: To study the effect of trasylol and tranexamic acid on alleviating degradation speed of fibrin glue stand. METHOD: We added trasylol and tranexamic acid to fibrin glue and form cell standard complex and cell streghtening stand complex, then conduct culture in vitro and observe reproduction situations of chondrocyte in stand, measurement of morphology and ultrastructure and degradation situations of carriers. RESULT: There were obvious differences in quality between two groups, after one or two weeks of culture in vitro. There were no significant differences in cell growth curve; Chondrocyte could secrete extracellular matrix after cubic culture in fibrin glue stand. CONCLUSION: Adding trasylol and tranexamic acid in fibrin glue can obviously alleviate the degradation speed of fibin glue and has no harmful effect on the reproduction of chondrocyte, sustainment of thenotype and secretation of matrix.

关 键 词：组织工程支架 纤维蛋白胶 抑肽酶 氨甲环酸 降解速度 软骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]