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作 者:张延滨[1] 孙连发[1] 辛文利[1] 宋庆杰[1] 张春利[1] 赵海滨[1] 肖志敏[1] 祁适雨[1]
机构地区:[1]黑龙江省农业科学院作物育种研究所,哈尔滨150086
出 处:《中国农业科学》2003年第3期242-247,共6页Scientia Agricultura Sinica
基 金:国家自然科学基金资助项目 ( 3 9770 461);黑龙江省攻关资助项目
摘 要:利用生化标记和选择性回交 (5~ 6次 )的方法将 5 +10亚基转移到黑龙江省主栽小麦品种克旱 9、克丰3、龙麦 2 0和垦大 4中。同一品种的 5 +10亚基类型与 2 +12或 3+12亚基类型相比 ,蛋白质和干面筋含量没有差别 (P >0 .1) ,湿面筋与干面筋的比值低 2 .9%~ 5 .0 % (P <0 .0 1) ,Zeleny沉降值与干面筋的比值高4 .5 %~ 13.4 %(P <0 .0 5 ) ,软化度低 15~ 2 5FU(P <0 .0 1) ,最大阻力高 82~ 193EU(P <0 .0 5 )。讨论了这种方法在改造现有小麦主栽品种中的应用。HMW GS 5+10 was introduced to four major wheat cultivars Kehan9, Kefeng3, Longmai20 and Kenda4 through 5-6 consecutive backcrosses and biochemical marker assisted selection. No significant difference in protein content and dry gluten content was observed between cultivars containing 5+10 and 2+12 or 3+12 (P>0.1). In cultivars containing HMW GS 5+10, the ratio of wet gluten content to dry gluten content to dry gluten was 2.9%-5.0% (P<0.01) lower than the ratio from the cultivars containing 2+12 or 3+12, but the ratio of Zeleny sedimentation volume to dry gluten content is 4.5%-13.4% (P<0.05) higher. The degree of softening in cultivars having 5+10 was 15-25 FU (P<0.01) lower, while the maximum resistance was 82-193 EU (P<0.05) higher. The application of consecutive backcrosses and biochemical marker assisted selection in the improvement of quality of in wheat cultivars was discussed.
关 键 词:品种 品质改良 小麦 高分子量麦谷蛋白亚基 选择性回交
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