水稻稻瘟病菌胁迫应答cDNA片段的表达及定位  

Expression and Mapping of cDNA Fragments Responsive to Magnaporthe grisea in Rice

作　　者：饶志明[1] 董海涛[2] 庄杰云[1] 柴荣耀[3] 樊叶杨[1] 李德葆[2] 郑康乐[1] 

机构地区：[1]中国水稻研究所国家水稻改良中心,浙江杭州310006 [2]浙江大学农业部植物病理学重点实验室,浙江杭州310029 [3]浙江省农业科学院植物保护研究所,浙江杭州310021

出　　处：《中国水稻科学》2003年第2期105-108,共4页Chinese Journal of Rice Science

基　　金：浙江省自然科学基金资助项目 ( 3 0 0 0 0 6) ;国家自然科学基金资助项目 ( 3 0 0 70 40 0 ) ;农业部水稻生物学重点开放实验室资助项目 ( 0 0 0 2 0 3 )

摘　　要：以抗稻瘟病品系 G2 0 5为材料 ,应用 c DNA微阵列分别获得了一个受稻瘟病菌诱导的含 NBS- L RR的 c DNA克隆 (暂命名为 RIM1,rice induced by Magnaporthe grisea)和一个受稻瘟病菌抑制的编码腈水解酶 (Nitrilase)的 c DNA克隆(暂命名为 N IT) ,并通过 Northern得到证实。RFLP分析将 RIM1和 N IT分别定位于水稻第 2和第 3染色体上 ,它们均位于控制水稻稻瘟病部分抗性The expression profiles of a resistant line G205 derived from a RIL population of Zhong 156×Gumei 2 in response to Magnaporthe grisea were investigated by using cDNA microarray containing 2200 expressed sequence tags (ESTs). One cDNA fragment encoding nucleotide binding site (NBS) and leucine rich repeat (LRR) was up regulated and the other cDNA fragment encoding nitrilase associated protein (NIT) was down regulated significantly by the fungus, which were confirmed by Northern blot. They were mapped on chromosomes 2 and 3 by RFLP analysis respectively. Both of them were located on the intervals where QTLs controlling partial resistance to blast had been detected.

关 键 词：水稻 CDNA微阵列 腈水解酶 基因定位 稻瘟病 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]