水稻种子萌动期抗低温逆境突变体的筛选  

Screen of rice mutants in adversity of low-temperature during germination.

作　　者：金静[1] 朱诚[1] 傅亚萍[2] 孙宗修[2] 

机构地区：[1]浙江大学生物科学系,浙江杭州310029 [2]中国水稻研究所农业部水稻生物学重点实验室,浙江杭州310006

出　　处：《浙江大学学报（农业与生命科学版）》2003年第2期207-209,共3页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金重点资助项目(3978020);国家973计划资助项目(C199901160)

摘　　要：利用粳稻(OryzasativaL.subsp.japonica)中花11构建的转座子突变体库,进行了水稻种子萌动期抗低温逆境突变体的筛选。以不同年份的种子为材料,在低温下观察种子的发芽率。结果表明,在低温下粳稻突变体(编号T108A5和T237b1)种子的发芽率比对照亲本中花11高出近50%,同一株系不同年份种子的两次发芽率具有相关性,说明这种筛选耐低温突变体的方法是可行的,在分子水平上的进一步检测正在进行中。The screen of rice mutants in adversity of temperature out from a tran sposon Ac/Ds inserted rice transgenic population during germination was performe d. Under low temperature, we observed the germination of seeds harvested in diff erent years and found that the mutants (NO. T108A5 and T237b1) were higher than the control by approximate 50%. Germination relativity between the same seeds ga ined in two years demonstrated the method of screen mutants was available and fu rther researches haves been going along.

关 键 词：水稻 ORYZA satiua L.subsp.japonica 种子 抗低温逆境 突变体 

分 类 号：S351.52[农业科学—作物栽培与耕作技术]