慢性丙型肝炎患者HCV准种的变化及其意义  

Change and significance of HCV quasispecies in patients with chronic hepatitis C.

作　　者：田辉[1] 王占英[2] 李颖[2] 黄芬[2] 刘沛[2] 

机构地区：[1]110034,沈阳,解放军第二四二医院感染科 [2]中国医科大学第二临床学院感染科

出　　处：《肝脏》2003年第1期12-14,共3页Chinese Hepatology

摘　　要：目的　探讨HCV准种与丙型肝炎慢性化的关系。方法　以 3 0例HCVRNA(HVR1区PCR )阳性的丙型肝炎患者为研究对象 ,采用单链构象多态性分析 (SSCP)进行HCV准种检测。结果　 8例急性丙型肝炎、15例慢性丙型肝炎和 7例肝硬化患者的SSCP条带数分别为 ( 3 .13± 1.0 7)、( 5 .0 7± 1.48)和 ( 5 .5 7± 2 .15 ) ,慢性肝炎组、肝硬化组与急性肝炎组SSCP条带数比较有显著差异 (P <0 .0 5 )。通过输血或使用血制品感染HCV者共 17例 ,SSCP条带数为 ( 5 .3± 1.8) ,散发感染组 11例 ,SSCP条带数为 ( 3 .9± 1.6) ,两组比较有显著差异 (P <0 .0 5 )。 2 2例慢性丙型肝炎和肝硬化患者的SSCP条带数与病程相关系数分别为 0 .5 72 (Pearson’s相关 )和 0 .5 5 6(Spearman’s相关 ) ,与性别、年龄、ALT水平无相关性。结论　HCV准种数量与丙型肝炎慢性化密切相关 ;HCV准种数量与丙型肝炎感染途径相关 ,输血或血制品感染者HCV准种数量增多 ;HCV准种数量与病程呈正相关 ,感染时间长 ,HCV准种数量多。Objective To explore the relationship between HCV quasispecies and persistent HCV infection. Methods HCV quasispecies from 30 patients of hepatitis C with positive serum HCVRNA(HVR1) was detected by single strand conformation polymorphism (SSCP) analysis.Results 3.13±1.07,5.07±1.48 and 5.57±2.15 SSCP bands were detected in 8 patients with acute hepatitis, 15 with chronic hepatitis and 7 patients with liver cirrhosis, respectively. The number of viral quasispecies in the patients with chronic hepatitis and liver cirrhosis was significantly higher than that in the patients with acute hepatitis C( P <0.05). The number of SSCP bands in 17 patients whose infection was caused by blood transfusion or blood products was 5.3±1.8, the number of SSCP bands in 11 patients whose infection acquired by sporadic infection was 3.9±1.6, the difference between the posttransfusion and sporadic hepatitis groups was significant ( P <0.05). No correlation was found between the number of quasispecies and gender, age or the levels of serum ALT in the 22 patients with chronic hepatitis and cirrhosis, but the Pearson's and Spearman's rank correlation coefficient between the number of SSCP bands and the duration was 0.572, 0.556 respectively ( P <0.01).Conclusions HCV quasispecies is strongly related to chronicity of HCV infection. The number of HCV quasispecies correlates with transmission routes. Patients who acquire HCV by blood transfusion or blood products have a greater number of HCV quasispecies. The number of HCV quasispecies correlates with the duration of HCV infection.

关 键 词：慢性丙型肝炎 HCV 准种 单链构象多态性分析 

分 类 号：R512.63[医药卫生—内科学]