条件反射性抗体反应增强的动态分析——以OVA为非条件刺激物  

THE DYNAMIC ANALYSIS OF CONDITIONED ENHANCEMENT OF ANTIBODY RESPONSE TO OVALBUMIN

作　　者：陈极寰[1] 林文娟[1] 王玮雯[1] 杨杰[1] 邵枫[1] 

机构地区：[1]中国科学院心理研究所脑-行为研究中心,北京100101

出　　处：《心理学报》2003年第2期261-265,共5页Acta Psychologica Sinica

基　　金：国家自然科学基金资助项目(批准号:39830130;39770265);中国科学院知识创新工程资助项目(批准号:KSCX2-2-03)。

摘　　要：研究了条件反射性抗体反应增强模型的建立。被试为49只雄性成年Wistar大鼠,采用糖精水作为条件性刺激,一种蛋白抗原卵清蛋白作为非条件性刺激配对给予大鼠,两者结合后,在初次抗体应答下降阶段再次单独给予条件刺激,用酶联免疫吸附法分时段检测抗体水平的变化。发现条件组在条件刺激后15,20,25天左右抗体水平明显高于对照组。这一过程与初次抗体应答的规律类似。这些结果证实经一次条件训练,单独给予条件刺激能诱导出明显的条件反射性抗体反应增高。To establish the model of conditioned enhancement of antibody production. Forty - nine male and adult Wistar rats were used. A novel taste of saccharin was served as the conditioned stimulus (CS) and an injection of a protein antigen ovalbumin as the unconditioned stimulus ( UCS) . After a CS/UCS pairing was made, animals were re - exposed to the CS alone. Blood samples were periodically collected by tail incisions after re - exposure to the CS. The level of anti - OVA antibody production was measured by enzyme linked immunosorbent assay (ELISA). The results showed that the levels of anti - OVA antibody production of the group CS were significantly greater than the controls at the day 15 , 20 and 25 after the CS presentation. The pattern of the conditioned anti - OVA IgG production was similar to that of the primary antibody response. The results reinforced the view that the enhancement of antibody production can be induced by single - trial Pavlovian conditioning where the CS alone is sufficient.

关 键 词：条件反射性免疫增强 动态分析 卵清蛋白 大鼠 

分 类 号：B845[哲学宗教—心理学]